1g8u
From Proteopedia
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|PDB= 1g8u |SIZE=350|CAPTION= <scene name='initialview01'>1g8u</scene>, resolution 1.85Å | |PDB= 1g8u |SIZE=350|CAPTION= <scene name='initialview01'>1g8u</scene>, resolution 1.85Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=UFR:2'-DEOXY-5-FORMYLURIDINE+5'-(DIHYDROGEN+PHOSPHATE)'>UFR</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1g8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g8u OCA], [http://www.ebi.ac.uk/pdbsum/1g8u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1g8u RCSB]</span> | ||
}} | }} | ||
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[[Category: Tsunoda, M.]] | [[Category: Tsunoda, M.]] | ||
[[Category: Ueno, Y.]] | [[Category: Ueno, Y.]] | ||
| - | [[Category: b-dna]] | ||
[[Category: deoxyribonucleic acid]] | [[Category: deoxyribonucleic acid]] | ||
| - | [[Category: dna damage]] | + | [[Category: dna damage,b-dna]] |
[[Category: double helix]] | [[Category: double helix]] | ||
[[Category: formyluridine]] | [[Category: formyluridine]] | ||
[[Category: modified nucleotide]] | [[Category: modified nucleotide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:39:02 2008'' |
Revision as of 17:39, 30 March 2008
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| , resolution 1.85Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATF5UCGCG):5-FORMYLURIDINE/ ADENOSINE BASE-PAIRS IN B-DNA
Overview
To investigate the role of divalent cations in crystal packing, four different crystals of a Dickerson-type dodecamer with the sequence d(CGCGAATXCGCG), containing 2'-deoxy-5-formyluridine at X, were obtained under several conditions with and without divalent cations. The crystal structures are all isomorphous. The octahedrally hydrated magnesium cations found in the major groove cement the two neighbouring duplexes along the b axis. In the Mg(2+)-free crystals, a five-membered ring of water molecules occupies the same position as the magnesium site and connects the two duplexes similarly to the hydrated Mg(2+) ion. It has been concluded that water molecules can take the place of the hydrated magnesium cation in crystallization, but the magnesium cation is more effective and gives X-ray diffraction at slightly higher resolution. In all four crystals, the 5-formyluracil residues form the canonical Watson-Crick pair with adenine residues.
About this Structure
1G8U is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystallization and preliminary X-ray analysis of a DNA dodecamer containing 2'-deoxy-5-formyluridine; what is the role of magnesium cation in crystallization of Dickerson-type DNA dodecamers?, Tsunoda M, Karino N, Ueno Y, Matsuda A, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):345-8. PMID:11173500
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