Exonuclease
From Proteopedia
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'''Exonucleases''' (ExN) cleave nucleotides at the end of a polynucleotide chain<ref>PMID:14770007</ref>. In ''E. coli'' there are ExN I to VIII. ExN were found in human and yeast as well. | '''Exonucleases''' (ExN) cleave nucleotides at the end of a polynucleotide chain<ref>PMID:14770007</ref>. In ''E. coli'' there are ExN I to VIII. ExN were found in human and yeast as well. | ||
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* '''ExN-I''' cleaves single-stranded DNA.<br /> | * '''ExN-I''' cleaves single-stranded DNA.<br /> | ||
* '''ExN-II''' is associated with DNA polymerase.<br /> | * '''ExN-II''' is associated with DNA polymerase.<br /> | ||
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* '''ExN-V''' cleaves linear double-stranded DNA.<br /> | * '''ExN-V''' cleaves linear double-stranded DNA.<br /> | ||
* '''ExN-VIII''' requires a free 5’ OH group for reacting.<br /> | * '''ExN-VIII''' requires a free 5’ OH group for reacting.<br /> | ||
| + | * For '''phage ExN''' see [[Sand box 211]]. | ||
<scene name='46/466467/Cv/2'>Mg coordination site</scene> in ''E. coli'' exonuclease I (PDB code [[1fxx]]).<ref>PMID:11101894</ref> Water molecules shown as red spheres. | <scene name='46/466467/Cv/2'>Mg coordination site</scene> in ''E. coli'' exonuclease I (PDB code [[1fxx]]).<ref>PMID:11101894</ref> Water molecules shown as red spheres. | ||
Revision as of 10:24, 21 February 2016
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3D Structures of exonuclease
Updated on 21-February-2016
References
- ↑ Mukherjee D, Fritz DT, Kilpatrick WJ, Gao M, Wilusz J. Analysis of RNA exonucleolytic activities in cellular extracts. Methods Mol Biol. 2004;257:193-212. PMID:14770007 doi:http://dx.doi.org/10.1385/1-59259-750-5:193
- ↑ Breyer WA, Matthews BW. Structure of Escherichia coli exonuclease I suggests how processivity is achieved. Nat Struct Biol. 2000 Dec;7(12):1125-8. PMID:11101894 doi:10.1038/81978
