1gcj
From Proteopedia
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|PDB= 1gcj |SIZE=350|CAPTION= <scene name='initialview01'>1gcj</scene>, resolution 2.6Å | |PDB= 1gcj |SIZE=350|CAPTION= <scene name='initialview01'>1gcj</scene>, resolution 2.6Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ibr|1IBR]], [[1qgr|1QGR]], [[1qgk|1QGK]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gcj OCA], [http://www.ebi.ac.uk/pdbsum/1gcj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gcj RCSB]</span> | ||
}} | }} | ||
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[[Category: nuclear pore-targeting complex component]] | [[Category: nuclear pore-targeting complex component]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:41:21 2008'' |
Revision as of 17:41, 30 March 2008
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| , resolution 2.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Related: | 1IBR, 1QGR, 1QGK
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
N-TERMINAL FRAGMENT OF IMPORTIN-BETA
Overview
Importin-beta is a nuclear transport factor which mediates the nuclear import of various nuclear proteins. The N-terminal 1-449 residue fragment of mouse importin-beta (impbeta449) possesses the ability to bidirectionally translocate through the nuclear pore complex (NPC), and to bind RanGTP. The structure of the uncomplexed form of impbeta449 has been solved at a 2.6 A resolution by X-ray crystallography. It consists of ten copies of the tandemly arrayed HEAT repeat and exhibits conformational flexibility which is involved in protein-protein interaction for nuclear transport. The overall conformation of the HEAT repeats shows that a twisted motion produces a significantly varied superhelical architecture from the previously reported structure of RanGTP-bound importin-beta. These conformational changes appear to be the sum of small conformational changes throughout the polypeptide. Such a flexibility, which resides in the stacked HEAT repeats, is essential for interaction with RanGTP or with NPCs. Furthermore, it was found that impbeta449 has a structural similarity with another nuclear migrating protein, namely beta-catenin, which is composed of another type of helix-repeated structure of ARM repeat. Interestingly, the essential regions for NPC translocation for both importin-beta and beta-catenin are spatially well overlapped with one another. This strongly indicates the importance of helix stacking of the HEAT or ARM repeats for NPC-passage.
About this Structure
1GCJ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The adoption of a twisted structure of importin-beta is essential for the protein-protein interaction required for nuclear transport., Lee SJ, Imamoto N, Sakai H, Nakagawa A, Kose S, Koike M, Yamamoto M, Kumasaka T, Yoneda Y, Tsukihara T, J Mol Biol. 2000 Sep 8;302(1):251-64. PMID:10964573
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