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Sandbox Wabash 27 Fumarase
From Proteopedia
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<StructureSection load='1YFE' size='340' side='right' caption='Fumarase' scene=''> | <StructureSection load='1YFE' size='340' side='right' caption='Fumarase' scene=''> | ||
Fumarase (fumarate hydratase) is an enzyme found in eukaryotic cells which catalyzes the reaction between L-malate and fumarate.<ref>PMID: 9098893</ref> The catalysis proceeds via the deprotination of the C3 carbon of L-malate, which is followed by the loss of the -OH group attached to the C2 carbon; the intermediate for which is a carbanion transition state.<ref>Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry. 3rd ed. New York: Wiley, 1999. Print.</ref> | Fumarase (fumarate hydratase) is an enzyme found in eukaryotic cells which catalyzes the reaction between L-malate and fumarate.<ref>PMID: 9098893</ref> The catalysis proceeds via the deprotination of the C3 carbon of L-malate, which is followed by the loss of the -OH group attached to the C2 carbon; the intermediate for which is a carbanion transition state.<ref>Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry. 3rd ed. New York: Wiley, 1999. Print.</ref> | ||
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== Active Site Characteristics == | == Active Site Characteristics == | ||
| - | Active Site-A: Site-A can be described as being comprised of atoms from three of the four sub-units ( | + | Active Site-A: Site-A can be described as being comprised of atoms from three of the four sub-units (tetramer). |
Active Site-B: | Active Site-B: | ||
== H188N == | == H188N == | ||
| - | H188N is used to refer to the fumarase C with five histidines on the C-terminal with histidine 188 mutated to asparagine<ref>PMID: 9098893< | + | H188N is used to refer to the fumarase C with five histidines on the C-terminal with histidine 188 mutated to asparagine<ref>PMID: 9098893<ref>. |
== H129N == | == H129N == | ||
| - | H129N is used to refer to the fumarase C with five histidines on the C-terminal with histidine 129 mutated to asparagine<ref>PMID: 9098893< | + | H129N is used to refer to the fumarase C with five histidines on the C-terminal with histidine 129 mutated to asparagine<ref>PMID: 9098893<ref>. |
== Summary and Conclusions == | == Summary and Conclusions == | ||
Revision as of 02:57, 28 February 2016
Fumarase: The Active Site Debate Answered
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References
- ↑ Weaver T, Lees M, Banaszak L. Mutations of fumarase that distinguish between the active site and a nearby dicarboxylic acid binding site. Protein Sci. 1997 Apr;6(4):834-42. PMID:9098893
- ↑ Voet, Donald, Judith G. Voet, and Charlotte W. Pratt. Fundamentals of Biochemistry. 3rd ed. New York: Wiley, 1999. Print.
- ↑ PMID: 9098893<ref>. == H129N == H129N is used to refer to the fumarase C with five histidines on the C-terminal with histidine 129 mutated to asparagine<ref>PMID: 9098893<ref>. == Summary and Conclusions == -Fumarase is a key component in the critic acid cycle - This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. </li></ol></ref>
