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Sandbox Wabash 18 Fumarase
From Proteopedia
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==The Active Site of Fumarase-McGue== | ==The Active Site of Fumarase-McGue== | ||
<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> | <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> | ||
| - | Fumarase is an enzyme that catalyzes the reactions of the hydration and dehydration of fumarate to malate, and vice versa. When the enzyme was first examined, there was a debate over the location of the active site of the enzyme. This confusion was due to the fact that there were two carboxylic acid binding sites on the fumarase that had the potential to be the active site. These binding sites were both present in wild type and inhibited forms of the enzyme. In order to determine which carboxylic acid binding site was the actual active site, mutants of these two binding sites were observed. | + | Fumarase is an enzyme that catalyzes the reactions of the hydration and dehydration of fumarate to malate, and vice versa. When the enzyme was first examined, there was a debate over the location of the active site of the enzyme. This confusion was due to the fact that there were two carboxylic acid binding sites on the fumarase that had the potential to be the active site. These binding sites were both present in wild type and inhibited forms of the enzyme. In order to determine which carboxylic acid binding site was the actual active site, mutants of these two binding sites were observed. The site that was designated "A", when it was mutated, resulted in a significant decrease in activity of the enzyme. On the other hand, the site designated "B" had no significant negative effect upon the activity of the fumarase. This indicates that the active site of fumarase is actually "A" because that is the binding site that the activity was affected by. |
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
Revision as of 00:33, 29 February 2016
The Active Site of Fumarase-McGue
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References
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
