1grz
From Proteopedia
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|PDB= 1grz |SIZE=350|CAPTION= <scene name='initialview01'>1grz</scene>, resolution 5.0Å | |PDB= 1grz |SIZE=350|CAPTION= <scene name='initialview01'>1grz</scene>, resolution 5.0Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1grz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1grz OCA], [http://www.ebi.ac.uk/pdbsum/1grz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1grz RCSB]</span> | ||
}} | }} | ||
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[[Category: rrna group i intron]] | [[Category: rrna group i intron]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:50:06 2008'' |
Revision as of 17:50, 30 March 2008
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| , resolution 5.0Å | |||||||
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| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A PREORGANIZED ACTIVE SITE IN THE CRYSTAL STRUCTURE OF THE TETRAHYMENA RIBOZYME
Overview
Group I introns possess a single active site that catalyzes the two sequential reactions of self-splicing. An RNA comprising the two domains of the Tetrahymena thermophila group I intron catalytic core retains activity, and the 5.0 angstrom crystal structure of this 247-nucleotide ribozyme is now described. Close packing of the two domains forms a shallow cleft capable of binding the short helix that contains the 5' splice site. The helix that provides the binding site for the guanosine substrate deviates significantly from A-form geometry, providing a tight binding pocket. The binding pockets for both the 5' splice site helix and guanosine are formed and oriented in the absence of these substrates. Thus, this large ribozyme is largely preorganized for catalysis, much like a globular protein enzyme.
About this Structure
1GRZ is a Protein complex structure of sequences from Tetrahymena thermophila. Full crystallographic information is available from OCA.
Reference
A preorganized active site in the crystal structure of the Tetrahymena ribozyme., Golden BL, Gooding AR, Podell ER, Cech TR, Science. 1998 Oct 9;282(5387):259-64. PMID:9841391
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