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1gvg
From Proteopedia
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|PDB= 1gvg |SIZE=350|CAPTION= <scene name='initialview01'>1gvg</scene>, resolution 1.54Å | |PDB= 1gvg |SIZE=350|CAPTION= <scene name='initialview01'>1gvg</scene>, resolution 1.54Å | ||
|SITE= <scene name='pdbsite=AC1:Nmo+Binding+Site+For+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Nmo+Binding+Site+For+Chain+A'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=AKG:2-OXYGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NO:NITROGEN+OXIDE'>NO</scene>, <scene name='pdbligand=PCX:DEOXYGUANIDINOPROCLAVAMINIC+ACID'>PCX</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gvg OCA], [http://www.ebi.ac.uk/pdbsum/1gvg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gvg RCSB]</span> | ||
}} | }} | ||
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[[Category: Schofield, C J.]] | [[Category: Schofield, C J.]] | ||
[[Category: Zhang, Z H.]] | [[Category: Zhang, Z H.]] | ||
| - | [[Category: AKG]] | ||
| - | [[Category: FE]] | ||
| - | [[Category: NO]] | ||
| - | [[Category: PCX]] | ||
| - | [[Category: SO4]] | ||
[[Category: clavaminate synthase 1]] | [[Category: clavaminate synthase 1]] | ||
[[Category: jelly roll]] | [[Category: jelly roll]] | ||
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[[Category: trifunctional enzyme]] | [[Category: trifunctional enzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:52:06 2008'' |
Revision as of 17:52, 30 March 2008
| |||||||
| , resolution 1.54Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF CLAVAMINATE SYNTHASE WITH NITRIC OXIDE
Overview
Clavaminate synthase (CAS), a 2-oxoglutarate (2OG) dependent dioxygenase, catalyses three steps in the biosynthesis of clavulanic acid. Crystals of CAS complexed with Fe(II), 2OG and deoxyguanidinoproclavaminate were exposed to nitric oxide (NO) acting as a dioxygen analogue. Prior to exposure with NO, the active site Fe(II) is octahedrally coordinated by a water molecule, the 2-oxo and 1-carboxylate groups of 2OG, and the side-chains of an aspartyl and two histidinyl residues. NO binds to the position previously occupied by the 2OG 1-carboxylate concomitant with rearrangement of the latter to the position previously occupied by the displaced water.
About this Structure
1GVG is a Single protein structure of sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA.
Reference
Crystal structure of a clavaminate synthase-Fe(II)-2-oxoglutarate-substrate-NO complex: evidence for metal centered rearrangements., Zhang Z, Ren J, Harlos K, McKinnon CH, Clifton IJ, Schofield CJ, FEBS Lett. 2002 Apr 24;517(1-3):7-12. PMID:12062399
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