1gwd
From Proteopedia
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|PDB= 1gwd |SIZE=350|CAPTION= <scene name='initialview01'>1gwd</scene>, resolution 1.77Å | |PDB= 1gwd |SIZE=350|CAPTION= <scene name='initialview01'>1gwd</scene>, resolution 1.77Å | ||
|SITE= <scene name='pdbsite=EA1:Egl+Binding+Site+For+Chain+A'>EA1</scene> | |SITE= <scene name='pdbsite=EA1:Egl+Binding+Site+For+Chain+A'>EA1</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gwd OCA], [http://www.ebi.ac.uk/pdbsum/1gwd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gwd RCSB]</span> | ||
}} | }} | ||
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[[Category: Bricogne, G.]] | [[Category: Bricogne, G.]] | ||
[[Category: Evans, G.]] | [[Category: Evans, G.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: CMO]] | ||
| - | [[Category: EDO]] | ||
| - | [[Category: IOD]] | ||
| - | [[Category: NA]] | ||
[[Category: aller]] | [[Category: aller]] | ||
[[Category: bacteriolytic enzyme]] | [[Category: bacteriolytic enzyme]] | ||
| Line 36: | Line 34: | ||
[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:52:40 2008'' |
Revision as of 17:52, 30 March 2008
| |||||||
| , resolution 1.77Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , | ||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TRI-IODIDE DERIVATIVE OF HEN EGG-WHITE LYSOZYME
Overview
A series of experiments performed at Cu Kalpha wavelength on in-house X-ray equipment are presented which investigate two possibilities for enhancing the experimental phasing signal by means of (i) triiodide/iodide soaks using KI/I(2) and (ii) combinations of counter-ions introduced using the short cryosoak method. Triiodide-derivative crystal structures for five test proteins have been refined and reveal that iodine can bind as polyiodide and single iodide ions through hydrophobic and hydrogen-bonding interactions both at the molecular surface and in intramolecular and intermolecular cavities. In three cases, the structures could be automatically determined with autoSHARP using in-house SAD and SIRAS data. The investigation of combinatorial counter-ion replacement using multiple salts with Na(+) and Cs(+) as cations and I(-) and Cl(-) as anions reveals that, for the case of hen egg-white lysozyme, significant improvement in phasing signal is obtained by the combined use of salts compared with SIRAS methods using native and single short-soak derivative data sets.
About this Structure
1GWD is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:12037300
Page seeded by OCA on Sun Mar 30 20:52:40 2008
