1gz8
From Proteopedia
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|PDB= 1gz8 |SIZE=350|CAPTION= <scene name='initialview01'>1gz8</scene>, resolution 1.30Å | |PDB= 1gz8 |SIZE=350|CAPTION= <scene name='initialview01'>1gz8</scene>, resolution 1.30Å | ||
|SITE= <scene name='pdbsite=MBP:Mbp+Binding+Site+For+Chain+A'>MBP</scene> | |SITE= <scene name='pdbsite=MBP:Mbp+Binding+Site+For+Chain+A'>MBP</scene> | ||
| - | |LIGAND= <scene name='pdbligand=MBP:1-[(2-AMINO-6,9-DIHYDRO-1H-PURIN-6-YL)OXY]-3-METHYL-2-BUTANOL'>MBP</scene> | + | |LIGAND= <scene name='pdbligand=CSW:CYSTEINE-S-DIOXIDE'>CSW</scene>, <scene name='pdbligand=MBP:1-[(2-AMINO-6,9-DIHYDRO-1H-PURIN-6-YL)OXY]-3-METHYL-2-BUTANOL'>MBP</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/ | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gz8 OCA], [http://www.ebi.ac.uk/pdbsum/1gz8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gz8 RCSB]</span> | ||
}} | }} | ||
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Probing the ATP ribose-binding domain of cyclin-dependent kinases 1 and 2 with O(6)-substituted guanine derivatives., Gibson AE, Arris CE, Bentley J, Boyle FT, Curtin NJ, Davies TG, Endicott JA, Golding BT, Grant S, Griffin RJ, Jewsbury P, Johnson LN, Mesguiche V, Newell DR, Noble ME, Tucker JA, Whitfield HJ, J Med Chem. 2002 Aug 1;45(16):3381-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12139449 12139449] | Probing the ATP ribose-binding domain of cyclin-dependent kinases 1 and 2 with O(6)-substituted guanine derivatives., Gibson AE, Arris CE, Bentley J, Boyle FT, Curtin NJ, Davies TG, Endicott JA, Golding BT, Grant S, Griffin RJ, Jewsbury P, Johnson LN, Mesguiche V, Newell DR, Noble ME, Tucker JA, Whitfield HJ, J Med Chem. 2002 Aug 1;45(16):3381-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12139449 12139449] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| + | [[Category: Non-specific serine/threonine protein kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Transferred entry: 2 7.11 1]] | ||
[[Category: Davies, T.]] | [[Category: Davies, T.]] | ||
[[Category: Endicott, J.]] | [[Category: Endicott, J.]] | ||
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[[Category: Noble, M.]] | [[Category: Noble, M.]] | ||
[[Category: Tucker, J.]] | [[Category: Tucker, J.]] | ||
| - | [[Category: MBP]] | ||
[[Category: 3d-structure.]] | [[Category: 3d-structure.]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:54:26 2008'' |
Revision as of 17:54, 30 March 2008
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| , resolution 1.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , | ||||||
| Activity: | Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN CYCLIN DEPENDENT KINASE 2 COMPLEXED WITH THE INHIBITOR 2-AMINO-6-(3'-METHYL-2'-OXO)BUTOXYPURINE
Overview
O(6)-substituted guanines are adenosine 5'-triphosphate (ATP) competitive inhibitors of CDK1/cyclin B1 and CDK2/cyclin A, the O(6) substituent occupying the kinase ribose binding site. Fifty-eight O(6)-substituted guanines were prepared to probe the ribose pocket, and the structures of four representative compounds bound to monomeric CDK2 were determined by X-ray crystallography. Optimum binding occurs with a moderately sized aliphatic O(6) substituent that packs tightly against the hydrophobic patch presented by the glycine loop, centered on Val18, an interaction promoted by the conformational restraints imposed in a cyclohexylmethyl or cyclohexenylmethyl ring. Structure-based design generated (R)-(2-amino-9H-purin-6-yloxymethyl)pyrrolidin-2-one (56), which reproduces the reported hydrogen bonds formed between ATP and Asp86 and Gln131 but failed to improve inhibitory potency. Thus, the parent compound O(6)-cyclohexylmethylguanine (NU2058, 25) is the preferred starting point for exploring other areas of the kinase active site.
About this Structure
1GZ8 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Probing the ATP ribose-binding domain of cyclin-dependent kinases 1 and 2 with O(6)-substituted guanine derivatives., Gibson AE, Arris CE, Bentley J, Boyle FT, Curtin NJ, Davies TG, Endicott JA, Golding BT, Grant S, Griffin RJ, Jewsbury P, Johnson LN, Mesguiche V, Newell DR, Noble ME, Tucker JA, Whitfield HJ, J Med Chem. 2002 Aug 1;45(16):3381-93. PMID:12139449
Page seeded by OCA on Sun Mar 30 20:54:26 2008
Categories: Homo sapiens | Non-specific serine/threonine protein kinase | Single protein | Davies, T. | Endicott, J. | Johnson, L. | Noble, M. | Tucker, J. | 3d-structure. | Atp-binding | Cell cycle | Cell division | Inhibition | Mitosis | Phosphorylation | Protein kinase | Serine/threonine-protein kinase | Transferase
