Sandbox Reserved 1176

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This Sandbox is Reserved from Jan 11 through August 12, 2016 for use in the course CH462 Central Metabolism taught by R. Jeremy Johnson at the Butler University, Indianapolis, USA. This reservation includes Sandbox Reserved 1160 through Sandbox Reserved 1184.

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Rattus norevegicus NTSR1

This is a default text for your page Allison Cotter/Sandbox 1. Click above on edit this page to modify. Be careful with the < and > signs.

You may include any references to papers as in: the use of JSmol in Proteopedia ^[1] or to the article describing Jmol ^[2] to the rescue.

1 Introduction
2 Structure
3 Biological Relevance
4 Structural Research
- 4.1 Active-Like State
- 4.2 Active State

Introduction

Neurotensin receptor 1 (NTSR1) is a G-protein coupled receptor (GPCR) that binds to the 13 amino acid peptide, neurotensin. Studies determining the structure of NTSR1 crystallized the GPCR bound with the C-terminus of its tridecapeptide ligand, because it has a higher potency and efficacy than its full-length counterpart. NTSR1 is a class A GPCR, and like all G-proteins, consists of an extracellular binding domain along with 7 transmembrane helices. Along with the ligand binding pocket at the top of the protein, NTSR1 also contains an allosteric Na+ ion binding pocket underneath. NTS binds to NTSR1, leading to a conformational change of the protein and modulation of second messengers. NTS has been shown to have a variety of biological activities including a role in the leptin signalling pathways, tumor growth, and dopamine regulation. The majority of effects of NTS are mediated through NTSR1. Research of the structure of NTSR1 has focused on the differences between its active and active-like states.

Structure

Ligand Binding Pocket

Near the top of the protein is the This pocket is comprised of several key amino acid resides. The first reside is a Phenylalanine at position F358. The purpose of this amino acid is to take part in a network of hydrophobic stacking interactions. These interactions stabilize the Y324 and W321 residues. These are crucial interactions because the Y324 is the amino acid residue that directly interacts with the via https://en.wikipedia.org/wiki/Van_der_Waals_force Van der Waals interactions. Without the hydrophobic stacking interactions that are facilitated by the F358 this binding interaction would not occur. The W321 residue also partakes in these stacking interactions. The W321 serves as the boundary between the ligand binding pocket and the sodium binding pocket.

Na+ Binding Pocket

, which is positioned at the bottom of the hydrophobic pocket(green link from allie), sets the top of the . The Na+ ion binding pocket acts as a negative allosteric site for G protein activity. When Na+ enters the Na+ ion binding pocket, it coordinates with Asp95, Gln131, and S135, and shuts down the activity of the protein. When the G protein is in its active state, the Na+ ion binding pocket is collapsed, preventing the regulation of protein activity through a Na+ ion. In this case, the Na+ ion is coordinated by a salt bridge to Asp113. The side chain atoms of Asp113 form a hydrogen bond network with Thr156, Ser361, Ser362, and Gln365, which prevents the coordination of a Na+ ion.

Neurotensin

is a 13 amino acid peptide that is found in both nervous and peripheral tissues. It functions as a hormone and a neurotransmitter by activating as the ligand for the G-protein coupled receptor NTSR1.

Biological Relevance

Leptin Research

A study was done by Liang Y. et al.to analyze the effects that being NTSR1 deficient had on mice. The results demonstrated that mice who were NTSR1 deficient were not able to receive a satiety signal.This resulted in the mice to continuing to eat as long as food was present, leading to significant weight gain. This is due to the fact that NTSR1 is involved in a signaling pathway that regulates leptin and therefore food intake. Without sufficient NTSR1 this pathway is interrupted. https://en.wikipedia.org/wiki/Leptin

Cancer Studies

It has been shown that some tumor cells can secrete and express Neurotensin and Neurotensin receptors themselves suggetsing that autocrine, endocrine and paracrine regulation by Neurotensin are possible. This leads to aggressive growth and possibly tumor development. A study was done that demonstrated that injecting animals with Neurotensin increased tumor growth and size, while injecting them with Neurotensin antagonist decreased tumor growth.It is believed that Neurotensin regulation may be used in future cancer treatment techniques.

Schizophrenia Research

The Dopamine Hypothesis http://www.schizophreniaforum.org/for/curr/AbiDargham/ states that having hyperdopamine levels may lead to schizophrenic symptoms. It has been shown that NTSR1 caused a blockade which inhibited firing in dopaminergic cells. It is believed that NTSR1 could therefore be used as a therapeutic for treating schizophrenia.Although this research is promising, the secondary effects were too extreme and the trial was discontinued.This is a pathway where NTSR1 research is focused on that could lead to ground breaking advances in treating schizophrenia.

Structural Research

Studies have been done to decipher the structure of NTSR1. Since wild type NTSR1 is unstable in detergent solution, select residues in the protein were mutated for stabilization. None of these residues are in the NTS binding pocket.

Active-Like State

Originally, six different amino acids were mutated for thermostabilization in detergent solution. These mutations were A86L, E166A, G215A, L310A, F358A, and V360A. This protein was found to have NTS affinity similar to that of wild tpye NTSR1, and was named NTSR1-GW5. Along with this, the Na+ ion binding pocket was found to be collapsed in this protein. However, studies found that NTSR1-GW5 did not have G-protein activity.

Active State

The active state protein

References

↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644

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 ==Structural Research==
-Studies have been done to decipher the exact structure of the NTSR1 protein. This data illustrated key residues that when mutated influence the activity of the protein. These mutations lead to the discovery of an  active state protein, an active-like protein state, and an inactive protein state depending on the specific amino acids that were mutated.
+Studies have been done to decipher the structure of NTSR1. Since wild type NTSR1 is unstable in detergent solution, select residues in the protein were mutated for stabilization. None of these residues are in the NTS binding pocket.
 ===Active-Like State===
+Originally, six different amino acids were mutated for thermostabilization in detergent solution. These mutations were A86L, E166A, G215A, L310A, F358A, and V360A. This protein was found to have NTS affinity similar to that of wild tpye NTSR1, and was named NTSR1-GW5. Along with this, the Na+ ion binding pocket was found to be collapsed in this protein. However, studies found that NTSR1-GW5 did not have G-protein activity.
 ===Active State===
 The active state protein