Kdo-8-phosphate synthase
From Proteopedia
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| - | {{STRUCTURE_3fyp| PDB=3fyp | SIZE=400| SCENE= |right|CAPTION=Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]] }} | + | {{STRUCTURE_3fyp| PDB=3fyp | SIZE=400| SCENE= |right|CAPTION=Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]] }} |
== Function == | == Function == | ||
'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). KPS is a metal-requiring enzyme. | '''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). KPS is a metal-requiring enzyme. | ||
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== Structural highlights == | == Structural highlights == | ||
The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>. | The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>. | ||
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| - | <ref>PMID:8528769</ref>. | ||
==3D structures of Kdo-8-phosphate synthase== | ==3D structures of Kdo-8-phosphate synthase== | ||
Revision as of 09:39, 5 April 2016
Contents |
Function
Kdo-8-phosphate synthase (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). KPS is a metal-requiring enzyme. Kdo-8-P is found in cell walls of higher plants and some green algae[1].
Structural highlights
The active site of the metallo KPS contains a divalent cation and the PEP substrate[2].
3D structures of Kdo-8-phosphate synthase
Updated on 05-April-2016
References
- ↑ Krosky DJ, Alm R, Berg M, Carmel G, Tummino PJ, Xu B, Yang W. Helicobacter pylori 3-deoxy-D-manno-octulosonate-8-phosphate (KDO-8-P) synthase is a zinc-metalloenzyme. Biochim Biophys Acta. 2002 Feb 11;1594(2):297-306. PMID:11904225
- ↑ Cochrane FC, Cookson TV, Jameson GB, Parker EJ. Reversing evolution: re-establishing obligate metal ion dependence in a metal-independent KDO8P synthase. J Mol Biol. 2009 Jul 24;390(4):646-61. Epub 2009 May 15. PMID:19447118 doi:10.1016/j.jmb.2009.05.014
