Introduction
Vitamin D Binding Protein (DPB) Overview:
1) purposes
2) History (various name changes since discovery)
3) overview of structure:
green scenes for , ball and stick, secondary structure
4) Brief mention of unique binding characteristics.
5) Therapeutic uses
Overall Structure
Basic Information
The secondary structure consists of mainly , which can be seen in pink. The quaternary structure of the protein consists of forming a complex.Due to “significant rotational freedom among the subdomains”[1] the two subunits when superimposed upon one another differ by about 6 degrees of rotation. The structure is about 52.1 kDA in size and made up of 458 amino acids.
Alpha Helical Domains
The Vitamin D binding protein consists of which are homologous to one another. containing 10 alpha helices, 9, and 4 being shorter than the other domains.
Vitamin D Binding Protein and Human Serum Albumin
The overall structure is closely related to that of the human serum albumin, to which it is homologous. The proteins are very similar yet the three dimensional structure differs somewhat to facilitate binding. The differences are due to bends at the C-terminal alpha-helices of the first and second domains in addition to rotations at the loops connecting the first two domains.
Actin Binding
The tertiary structure of the protein is optimized for it binding with actin, efficiently folding into a complex requiring little change of the structure. Once DPB binds to actin, the C-terminal alpha helix of the first domain and the loop between the second and third domain move to be in contact with the actin. The binding of actin to the differs from that of its homolog in the conformational changes that the protein undergoes, which can be attributed to differences in rotation in the first domain and the region between domain II III. Both green scenes are depicted with the same color scheme seen below.
Binding Interactions
The Vitamin D Binding Site
Describe the chemical make-up of the binding. Talk about hydrophobic interactions with vitamin D3 ligands. Hydrogen bond formation with 250HD3. Also discuss steric strain and binding affinity.
Biological Relevance of The Vitamin D Binding Site
Vitamin D hormone 1,25(OH)2D3 used to treat renal osteodystrophy, hypoparathyroidism and osteoporosis. Discuss selectivity and binding affinity of synthesized molecules
Additional Features
Vitamin D Binding Protein binds to many different different substrates including actin and various synthetic ligands
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Synthesis
Synthesized in the liver. Will also include details about folding, posttranslational modification and chaperone proteins, if any.
Actin Binding Interactions
Vitamin D binding protein is also capable of interacting with actin at the domains shown in . This function occurs mainly in the bloodstream, as DBP binds to globular actin present in the plasma. It presents an important mechanism for clearing actin from necrotic or apoptotic tissue (Meier et all 2006) scene name='48/483884/Dbp_actin_dbinding_distance/1'>TextToBeDisplayed</scene>
Other Interactions
"Macrophage modulation
Chemotaxis of C5 derived peptides
Transport of fatty acids and endotoxins
Inhibition of platelet induced aggregation
Osteoclast Activation" from Meier et al 2006 Figure 2
Role in Disease
Altered levels in hepatic failure, AHF, trauma, immune function.
Deficient mice show no phenotype. White and Cooke.
Other Notable Ligands
Quiz Question 1
. is very similar to based on sequence similarity as well as a similar tertiary structure. The two proteins can both bind to actin, however HSA is unable to bind to Vitamin D3. Based on what you have learned about the binding nature DBP, and looking at the structures of the two proteins, hypothesize a reason why HSA cannot bind to Vitamin D3. How can altering only a couple of amino acids so greatly alter the function and tertiary structure of proteins?
See Also
Credits
Introduction - Uday Prakhya
Overall Structure - Elizabeth Swanson
Drug Binding Site - Alex Debreceni
Additional Features - Nick Rivelli
Quiz Question 1 - Robert Green
References
- ↑ Verboven C, Rabijns A, De Maeyer M, Van Baelen H, Bouillon R, De Ranter C. A structural basis for the unique binding features of the human vitamin D-binding protein. Nat Struct Biol. 2002 Feb;9(2):131-6. PMID:11799400 doi:http://dx.doi.org/10.1038/nsb754
[1] Gomme PT, Bertolini J. 2004. Therapeutic potential of vitamin D-binding protein. Trends Biotechnol. 22:340–345.
[2] Haddad JG. 1995. Plasma vitamin D-binding protein (Gc-globulin): Multiple tasks. J. Steroid Biochem. Mol. Biol. 53:579–582.
[3] Otterbein LR, Cosio C, Graceffa P, Dominguez R. 2002. Crystal structures of the vitamin D-binding protein and its complex with actin: structural basis of the actin-scavenger system. Proc. Natl. Acad. Sci. U. S. A. 99:8003–8008.
[4] Speeckaert M, Huang G, Delanghe JR, Taes YEC. 2006. Biological and clinical aspects of the vitamin D binding protein (Gc-globulin) and its polymorphism. Clin. Chim. Acta 372:33–42.
[5] Verboven C, Rabijns A, De Maeyer M, Van Baelen H, Bouillon R, De Ranter C. 2002. A structural basis for the unique binding features of the human vitamin D-binding protein. Nat. Struct. Biol. 9:131–6.
[6] White P, Cooke N. 2000. The multifunctional properties and characteristics of vitamin D-binding protein. Trends Endocrinol. Metab. 11:320–327.
