Sandbox Reserved 427

Introduction

Vitamin D binding protein Overview: 1) purposes 2) History (various name changes since discovery) 3) overview of structure:

, ball and stick, secondary structure

4) Brief mention of unique binding characteristics. 5) Therapeutic uses

Overall Structure

Alpha Helical Domains

The Vitamin D binding protein consists of which are homologous to one another. containing 10 alpha helices, 9, and 4 being shorter than the other domains.

Vitamin D Binding Protein and Human Serum Albumin

The overall structure is closely related to that of the human serum albumin, to which it is homologous. The proteins are very similar yet the three dimensional structure differs somewhat to facilitate binding. The differences are due to bends at the C-terminal alpha-helices of the first and second domains in addition to rotations at the loops connecting the first two domains.

Actin Binding

The tertiary structure of the protein is optimized for it binding with actin, efficiently folding into a complex requiring little change of the structure.

Binding Interactions

The Vitamin D Binding Site

Describe the chemical make-up of the binding. Talk about hydrophobic interactions with vitamin D3 ligands. Hydrogen bond formation with 250HD3. Also discuss steric strain and binding affinity.

Biological Relevance of The Vitamin D Binding Site

Vitamin D hormone 1,25(OH)2D3 used to treat renal osteodystrophy, hypoparathyroidism and osteoporosis. Discuss selectivity and binding affinity of synthesized molecules

Additional Features

Vitamin D Binding Protein binds to many different different substrates including actin and various synthetic ligands scene='Insert optional scene name here' />

Synthesis

Synthesized in the liver. Will also include details about folding, posttranslational modification and chaperone proteins, if any.

Actin Binding Interactions

Vitamin D binding protein is also capable of interacting with actin at the domains shown in . This function occurs mainly in the bloodstream, as DBP binds to globular actin present in the plasma. It presents an important mechanism for clearing actin from necrotic or apoptotic tissue (Meier et all 2006) scene name='48/483884/Dbp_actin_dbinding_distance/1'>TextToBeDisplayed</scene>

Other Interactions

"Macrophage modulation Chemotaxis of C5 derived peptides Transport of fatty acids and endotoxins Inhibition of platelet induced aggregation Osteoclast Activation" from Meier et al 2006 Figure 2

Role in Disease

Altered levels in hepatic failure, AHF, trauma, immune function. Deficient mice show no phenotype. White and Cooke.

Other Notable Ligands

Quiz Question 1

. is very similar to based on sequence similarity as well as a similar tertiary structure. The two proteins can both bind to actin, however HSA is unable to bind to Vitamin D3. Based on what you have learned about the binding nature DBP, and looking at the structures of the two proteins, hypothesize a reason why HSA cannot bind to Vitamin D3. How can altering only a couple of amino acids so greatly alter the function and tertiary structure of proteins?

See Also

• 1kxp
• 1kw2
• 1j7e
• 1j78
• 1ma9
• 1lot

Credits

Introduction - Uday Prakhya

Overall Structure - Elizabeth Swanson

Drug Binding Site - Alex Debreceni

Additional Features - Nick Rivelli

Quiz Question 1 - Robert Green

References

1. ↑ Verboven C, Rabijns A, De Maeyer M, Van Baelen H, Bouillon R, De Ranter C. A structural basis for the unique binding features of the human vitamin D-binding protein. Nat Struct Biol. 2002 Feb;9(2):131-6. PMID:11799400 doi:http://dx.doi.org/10.1038/nsb754

[1] Gomme PT, Bertolini J. 2004. Therapeutic potential of vitamin D-binding protein. Trends Biotechnol. 22:340–345.

[2] Haddad JG. 1995. Plasma vitamin D-binding protein (Gc-globulin): Multiple tasks. J. Steroid Biochem. Mol. Biol. 53:579–582.

[3] Otterbein LR, Cosio C, Graceffa P, Dominguez R. 2002. Crystal structures of the vitamin D-binding protein and its complex with actin: structural basis of the actin-scavenger system. Proc. Natl. Acad. Sci. U. S. A. 99:8003–8008.

[4] Speeckaert M, Huang G, Delanghe JR, Taes YEC. 2006. Biological and clinical aspects of the vitamin D binding protein (Gc-globulin) and its polymorphism. Clin. Chim. Acta 372:33–42.

[5] Verboven C, Rabijns A, De Maeyer M, Van Baelen H, Bouillon R, De Ranter C. 2002. A structural basis for the unique binding features of the human vitamin D-binding protein. Nat. Struct. Biol. 9:131–6.

[6] White P, Cooke N. 2000. The multifunctional properties and characteristics of vitamin D-binding protein. Trends Endocrinol. Metab. 11:320–327.