1h7t
From Proteopedia
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|PDB= 1h7t |SIZE=350|CAPTION= <scene name='initialview01'>1h7t</scene>, resolution 2.48Å | |PDB= 1h7t |SIZE=350|CAPTION= <scene name='initialview01'>1h7t</scene>, resolution 2.48Å | ||
|SITE= <scene name='pdbsite=AC1:C-5'-MP+Binding+Site'>AC1</scene> and <scene name='pdbsite=AC2:O-Sialic+Acid+(Chair+conform.)+Bindin'>AC2</scene> | |SITE= <scene name='pdbsite=AC1:C-5'-MP+Binding+Site'>AC1</scene> and <scene name='pdbsite=AC2:O-Sialic+Acid+(Chair+conform.)+Bindin'>AC2</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=C5P:CYTIDINE-5'-MONOPHOSPHATE'>C5P</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate_cytidylyltransferase 3-deoxy-manno-octulosonate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.38 2.7.7.38] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-manno-octulosonate_cytidylyltransferase 3-deoxy-manno-octulosonate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.38 2.7.7.38] </span> |
|GENE= KPSU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= KPSU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h7t OCA], [http://www.ebi.ac.uk/pdbsum/1h7t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1h7t RCSB]</span> | ||
}} | }} | ||
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[[Category: Jelakovic, S.]] | [[Category: Jelakovic, S.]] | ||
[[Category: Schulz, G E.]] | [[Category: Schulz, G E.]] | ||
| - | [[Category: C5P]] | ||
| - | [[Category: SIA]] | ||
[[Category: cmp-kdo synthetase]] | [[Category: cmp-kdo synthetase]] | ||
[[Category: lipopolysaccharide biosynthesis]] | [[Category: lipopolysaccharide biosynthesis]] | ||
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[[Category: sugar-activating enzyme]] | [[Category: sugar-activating enzyme]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:59:33 2008'' |
Revision as of 17:59, 30 March 2008
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| , resolution 2.48Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | , | ||||||
| Gene: | KPSU (Escherichia coli) | ||||||
| Activity: | 3-deoxy-manno-octulosonate cytidylyltransferase, with EC number 2.7.7.38 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE STRUCTURE OF CMP:2-KETO-3-DEOXY-MANNO-OCTONIC ACID SYNTHETASE AND OF ITS COMPLEXES WITH SUBSTRATES AND SUBSTRATE ANALOGUES, HERE COMPLEX WITH CMP-NEUAC, CMP-NEUAC COMPLEX
Overview
The enzyme CMP-Kdo synthetase (CKS) catalyzes the activation of the sugar Kdo (2-keto-3-deoxy-manno-octonic acid) by forming a monophosphate diester. CKS is a pharmaceutical target because CMP-Kdo is used in the biosynthesis of lipopolysaccharides that are vital for Gram-negative bacteria. We have refined the structure of the unligated capsule-specific CKS from Escherichia coli at 1.8 A resolution (1 A=0.1 nm) and we have established the structures of its complexes with the substrate CTP, with CDP and CMP as well as with the product analog CMP-NeuAc (CMP-sialate) by X-ray diffraction analyses at resolutions between 2.1 A and 2.5 A. The N-terminal domains of the dimeric enzyme bind CTP in a peculiar nucleotide-binding fold, whereas the C-terminal domains form the dimer interface. The observed binding geometries together with the amino acid variabilities during evolution and the locations of a putative Mg(2+) and of a very strongly bound water molecule suggest a pathway for the catalysis. The N-terminal domain shows sequence homology with the CMP-NeuAc synthetases. Moreover, the chain fold and the substrate-binding position of CKS resemble those of other enzymes processing nucleotide-sugars.
About this Structure
1H7T is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The structure of CMP:2-keto-3-deoxy-manno-octonic acid synthetase and of its complexes with substrates and substrate analogs., Jelakovic S, Schulz GE, J Mol Biol. 2001 Sep 7;312(1):143-55. PMID:11545592
Page seeded by OCA on Sun Mar 30 20:59:33 2008
