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Mandelate dehydrogenase
From Proteopedia
(Difference between revisions)
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== Function == | == Function == | ||
'''S-mandelate dehydrogenase''' (SMD) catalyzes the conversion of S-2-hydroxy-2-phenylacetate and acceptor to 2-oxo-2-phenylacetate and reduced acceptor<ref>PMID:10231535</ref>. SMD is a FMN-dependent enzyme. '''D-mandelate dehydrogenase''' (DMD) catalyzes the NAD-dependent oxidation of D-mandalate to phenylglyoxylate. | '''S-mandelate dehydrogenase''' (SMD) catalyzes the conversion of S-2-hydroxy-2-phenylacetate and acceptor to 2-oxo-2-phenylacetate and reduced acceptor<ref>PMID:10231535</ref>. SMD is a FMN-dependent enzyme. '''D-mandelate dehydrogenase''' (DMD) catalyzes the NAD-dependent oxidation of D-mandalate to phenylglyoxylate. | ||
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| + | == Structural highlights == | ||
| + | The poor substrate indolelactate binds in the active site of SMD<ref>PMID:19465768</ref>. | ||
</StructureSection> | </StructureSection> | ||
==3D structures of mandelate dehydrogenase== | ==3D structures of mandelate dehydrogenase== | ||
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*S-mandelate dehydrogenase | *S-mandelate dehydrogenase | ||
| - | **[[1huv]], [[1p4c]], [[1p5b]], [[2a7n]], [[3giy]] – | + | **[[1huv]], [[1p4c]], [[1p5b]], [[2a7n]], [[3giy]] – PpSM + FMN - Pseudomonas putida<br /> |
| - | + | **[[2a7p]] – PpSMD (mutant) + FMN + 3-indolelacetate<br /> | |
| - | **[[2a7p]] – PpSMD (mutant) + 3-indolelacetate<br /> | + | **[[2a85]] – PpSMD (mutant) + FMN + 2-hydroxyoctanoate<br /> |
| - | **[[2a85]] – PpSMD (mutant) + 2-hydroxyoctanoate<br /> | + | |
*D-mandelate dehydrogenase | *D-mandelate dehydrogenase | ||
Revision as of 09:24, 17 April 2016
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3D structures of mandelate dehydrogenase
Updated on 17-April-2016
References
- ↑ Lehoux IE, Mitra B. (S)-Mandelate dehydrogenase from Pseudomonas putida: mechanistic studies with alternate substrates and pH and kinetic isotope effects. Biochemistry. 1999 May 4;38(18):5836-48. PMID:10231535 doi:http://dx.doi.org/10.1021/bi990024m
- ↑ Sukumar N, Dewanti A, Merli A, Rossi GL, Mitra B, Mathews FS. Structures of the G81A mutant form of the active chimera of (S)-mandelate dehydrogenase and its complex with two of its substrates. Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):543-52. Epub 2009, May 15. PMID:19465768 doi:10.1107/S0907444909010270
