Mannose-binding protein
From Proteopedia
(Difference between revisions)
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{{STRUCTURE_1kzb| PDB=1kzb | SIZE=400| SCENE= |right|CAPTION=Rat mannose-binding protein fragment complex with mannose (stick model) and Ca+2 ion, [[1kzb]] }} | {{STRUCTURE_1kzb| PDB=1kzb | SIZE=400| SCENE= |right|CAPTION=Rat mannose-binding protein fragment complex with mannose (stick model) and Ca+2 ion, [[1kzb]] }} | ||
== Function == | == Function == | ||
| - | '''Mannose-binding protein''' (MBL) is a lectin essential to innate immunity. MBL recognizes carbohydrate patterns like mannose or glucose located on the surface of microorganisms like bacteria, viruses and fungi. MBL binds to the microorganism and the activation of the lectin pathway ensues. MBL binds to apoptotic and senescent cells and enhances their engulfment by phagocytes. In the blood, MBL is bound to MBL-associated serine protease (MASP-2). Upon binding of MBL to a target mannose, the MASP initiates the complement cascade resulting in lysis of the pathogen<ref>PMID:14666735</ref>. | + | '''Mannose-binding protein''' (MBL) or '''Mannose-binding lectin''' or '''Mannan-binding lectin''' is a lectin essential to innate immunity. MBL recognizes carbohydrate patterns like mannose or glucose located on the surface of microorganisms like bacteria, viruses and fungi. MBL binds to the microorganism and the activation of the lectin pathway ensues. MBL binds to apoptotic and senescent cells and enhances their engulfment by phagocytes. In the blood, MBL is bound to MBL-associated serine protease (MASP-2). Upon binding of MBL to a target mannose, the MASP initiates the complement cascade resulting in lysis of the pathogen<ref>PMID:14666735</ref>. |
== Disease == | == Disease == | ||
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*'''mannose-binding protein''' | *'''mannose-binding protein''' | ||
| - | **[[1ytt]], [[1kwt]] - rMBL fragment - rat<br /> | + | **[[1ytt]], [[1kwt]], [[1nt0]] - rMBL fragment - rat<br /> |
| - | **[[1kmb]], [[1fif]] - rMBL fragment (mutant) | + | **[[1kmb]], [[1fif]] - rMBL fragment (mutant)<br /> |
| + | **[[1hup]] – hMBL – human<br /> | ||
| + | **[[3a0c]] – PcMBL – ''Polygonatum cyrtonema''<br /> | ||
| + | **[[4akd]] – MBL – ''Artocarpus integer''<br /> | ||
| + | **[[4okd]] – MsMBL – ''Mycobacterium smegmatis''<br /> | ||
| + | **[[1zhq]] – MvMBL – ''Microcystis viridis''<br /> | ||
| + | **[[2y1s]] – MaMBL – ''Microcystis aeruginosa'' - NMR<br /> | ||
*Mannose-binding protein complex with sugar | *Mannose-binding protein complex with sugar | ||
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**[[1kwy]], [[1kx1]], [[1kza]], [[1kzd]], [[1kze]] - rMBL fragment + bisaccharide<br /> | **[[1kwy]], [[1kx1]], [[1kza]], [[1kzd]], [[1kze]] - rMBL fragment + bisaccharide<br /> | ||
**[[1kwz]], [[1kx0]] - rMBL fragment (mutant) + bisaccharide<br /> | **[[1kwz]], [[1kx0]] - rMBL fragment (mutant) + bisaccharide<br /> | ||
| - | **[[1kzb]], [[1kzc]] - rMBL fragment + mannose | + | **[[1kzb]], [[1kzc]] - rMBL fragment + mannose<br /> |
| + | **[[1qmo]] – MBL + mannose – bean<br /> | ||
| + | **[[2chh]] – MBL + alpha-mannose + beta-mannose – ''Ralstonia solanacearum''<br /> | ||
| + | **[[3a0d]], [[3a0e]] – PcMBL + mannose derivative<br /> | ||
| + | **[[4oit]] – MsMBL + alpha-mannose + beta-mannose <br /> | ||
| + | **[[4oiz]] – MsMBL + methyl-mannose<br /> | ||
| + | **[[1zhs]] – MvMBL + ManGlcNac<br /> | ||
| + | **[[2yhh]] – MaMBL + alpha-mannose - NMR<br /> | ||
}} | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 09:10, 18 April 2016
Contents |
Function
Mannose-binding protein (MBL) or Mannose-binding lectin or Mannan-binding lectin is a lectin essential to innate immunity. MBL recognizes carbohydrate patterns like mannose or glucose located on the surface of microorganisms like bacteria, viruses and fungi. MBL binds to the microorganism and the activation of the lectin pathway ensues. MBL binds to apoptotic and senescent cells and enhances their engulfment by phagocytes. In the blood, MBL is bound to MBL-associated serine protease (MASP-2). Upon binding of MBL to a target mannose, the MASP initiates the complement cascade resulting in lysis of the pathogen[1].
Disease
MBL deficiency is associated with poor outcome of cystic fibrosis patients[2]. MBL deficiency is one of the most common human immunodeficiencies and arise primarily from 3 single point mutations in the MBL gene[3].
Structural highlights
MBL active site contains Ca+2 ion which coordinates with the mannose[4].
3D structures of mannose-binding protein
Updated on 18-April-2016
References
- ↑ Nakagawa T, Ma BY, Uemura K, Oka S, Kawasaki N, Kawasaki T. Role of mannan-binding protein, MBP, in innate immunity. Anticancer Res. 2003 Nov-Dec;23(6a):4467-71. PMID:14666735
- ↑ Carlsson M, Sjoholm AG, Eriksson L, Thiel S, Jensenius JC, Segelmark M, Truedsson L. Deficiency of the mannan-binding lectin pathway of complement and poor outcome in cystic fibrosis: bacterial colonization may be decisive for a relationship. Clin Exp Immunol. 2005 Feb;139(2):306-13. PMID:15654829 doi:http://dx.doi.org/10.1111/j.1365-2249.2004.02690.x
- ↑ Turner MW. The role of mannose-binding lectin in health and disease. Mol Immunol. 2003 Nov;40(7):423-9. PMID:14568388
- ↑ Ng KK, Kolatkar AR, Park-Snyder S, Feinberg H, Clark DA, Drickamer K, Weis WI. Orientation of bound ligands in mannose-binding proteins. Implications for multivalent ligand recognition. J Biol Chem. 2002 May 3;277(18):16088-95. Epub 2002 Feb 15. PMID:11850428 doi:10.1074/jbc.M200493200
