This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1hht
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1hht |SIZE=350|CAPTION= <scene name='initialview01'>1hht</scene>, resolution 2.9Å | |PDB= 1hht |SIZE=350|CAPTION= <scene name='initialview01'>1hht</scene>, resolution 2.9Å | ||
|SITE= <scene name='pdbsite=CA1:The+Three+Conserved+Active+Site+Aspartate'>CA1</scene>, <scene name='pdbsite=CA2:The+Three+Conserved+Active+Site+Aspartate'>CA2</scene>, <scene name='pdbsite=CA3:The+Three+Conserved+Active+Site+Aspartate'>CA3</scene>, <scene name='pdbsite=MN1:Mn+Binding+Site+For+Chain+A'>MN1</scene>, <scene name='pdbsite=MN2:Mn+Binding+Site+For+Chain+B'>MN2</scene> and <scene name='pdbsite=MN3:Mn+Binding+Site+For+Chain+C'>MN3</scene> | |SITE= <scene name='pdbsite=CA1:The+Three+Conserved+Active+Site+Aspartate'>CA1</scene>, <scene name='pdbsite=CA2:The+Three+Conserved+Active+Site+Aspartate'>CA2</scene>, <scene name='pdbsite=CA3:The+Three+Conserved+Active+Site+Aspartate'>CA3</scene>, <scene name='pdbsite=MN1:Mn+Binding+Site+For+Chain+A'>MN1</scene>, <scene name='pdbsite=MN2:Mn+Binding+Site+For+Chain+B'>MN2</scene> and <scene name='pdbsite=MN3:Mn+Binding+Site+For+Chain+C'>MN3</scene> | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1hhs|1HHS]], [[1hi0|1HI0]], [[1hi1|1HI1]], [[1hi8|1HI8]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hht OCA], [http://www.ebi.ac.uk/pdbsum/1hht PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hht RCSB]</span> | ||
}} | }} | ||
| Line 16: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1HHT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1HHT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HHT OCA]. |
==Reference== | ==Reference== | ||
A mechanism for initiating RNA-dependent RNA polymerization., Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI, Nature. 2001 Mar 8;410(6825):235-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11242087 11242087] | A mechanism for initiating RNA-dependent RNA polymerization., Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI, Nature. 2001 Mar 8;410(6825):235-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11242087 11242087] | ||
| - | [[Category: | + | [[Category: Pseudomonas phage phi6]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bamford, D H.]] | [[Category: Bamford, D H.]] | ||
| Line 27: | Line 30: | ||
[[Category: Makeyev, E V.]] | [[Category: Makeyev, E V.]] | ||
[[Category: Stuart, D I.]] | [[Category: Stuart, D I.]] | ||
| - | [[Category: MN]] | ||
[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
[[Category: viral polymerase]] | [[Category: viral polymerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:05:10 2008'' |
Revision as of 18:05, 30 March 2008
| |||||||
| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , and | ||||||
| Ligands: | , , | ||||||
| Related: | 1HHS, 1HI0, 1HI1, 1HI8
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS TEMPLATE
Overview
In most RNA viruses, genome replication and transcription are catalysed by a viral RNA-dependent RNA polymerase. Double-stranded RNA viruses perform these operations in a capsid (the polymerase complex), using an enzyme that can read both single- and double-stranded RNA. Structures have been solved for such viral capsids, but they do not resolve the polymerase subunits in any detail. Here we show that the 2 A resolution X-ray structure of the active polymerase subunit from the double-stranded RNA bacteriophage straight phi6 is highly similar to that of the polymerase of hepatitis C virus, providing an evolutionary link between double-stranded RNA viruses and flaviviruses. By crystal soaking and co-crystallization, we determined a number of other structures, including complexes with oligonucleotide and/or nucleoside triphosphates (NTPs), that suggest a mechanism by which the incoming double-stranded RNA is opened up to feed the template through to the active site, while the substrates enter by another route. The template strand initially overshoots, locking into a specificity pocket, and then, in the presence of cognate NTPs, reverses to form the initiation complex; this process engages two NTPs, one of which acts with the carboxy-terminal domain of the protein to prime the reaction. Our results provide a working model for the initiation of replication and transcription.
About this Structure
1HHT is a Single protein structure of sequence from Pseudomonas phage phi6. Full crystallographic information is available from OCA.
Reference
A mechanism for initiating RNA-dependent RNA polymerization., Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI, Nature. 2001 Mar 8;410(6825):235-40. PMID:11242087
Page seeded by OCA on Sun Mar 30 21:05:10 2008
