1hkh
From Proteopedia
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|PDB= 1hkh |SIZE=350|CAPTION= <scene name='initialview01'>1hkh</scene>, resolution 1.73Å | |PDB= 1hkh |SIZE=350|CAPTION= <scene name='initialview01'>1hkh</scene>, resolution 1.73Å | ||
|SITE= <scene name='pdbsite=CA:So4+Binding+Site+For+Chain+B'>CA</scene> | |SITE= <scene name='pdbsite=CA:So4+Binding+Site+For+Chain+B'>CA</scene> | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkh OCA], [http://www.ebi.ac.uk/pdbsum/1hkh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hkh RCSB]</span> | ||
}} | }} | ||
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[[Category: Line, K.]] | [[Category: Line, K.]] | ||
[[Category: Littlechild, J A.]] | [[Category: Littlechild, J A.]] | ||
| - | [[Category: SO4]] | ||
[[Category: alpha/beta hydrolase]] | [[Category: alpha/beta hydrolase]] | ||
| - | [[Category: co-factor free haloperoxidase]] | + | [[Category: co-factor free haloperoxidase,]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:06:39 2008'' |
Revision as of 18:06, 30 March 2008
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| , resolution 1.73Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
UNLIGATED GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES
Overview
The structure of the recombinant (-) gamma-lactamase from an Aureobacterium species has been solved at 1.73A resolution in the cubic space group F23 with unit cell parameters a=b=c=240.6A. The trimeric enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor free haloperoxidases. The structure has been solved in complex with a covalently bound ligand originating from the host cell and also in the unligated form. The associated density in the former structure has been interpreted as the two-ring ligand (3aR,7aS)-3a,4,7,7a-tetrahydro-benzo [1,3] dioxol-2-one which forms a tetrahedral complex with OG of the catalytic Ser98. Soaks of these crystals with the industrial substrate gamma-lactam or its structural analogue, norcamphor, result in the displacement of the ligand from the enzyme active site, thereby allowing determination of the unligated structure. The presence of the ligand in the active site protects the enzyme from serine hydrolase inhibitors. Cyclic ethylene carbonate, the first ring of the ligand, was shown to be a substrate of the enzyme.
About this Structure
1HKH is a Single protein structure of sequence from Microbacterium. Full crystallographic information is available from OCA.
Reference
The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site., Line K, Isupov MN, Littlechild JA, J Mol Biol. 2004 Apr 30;338(3):519-32. PMID:15081810
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