1ht9
From Proteopedia
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|PDB= 1ht9 |SIZE=350|CAPTION= <scene name='initialview01'>1ht9</scene>, resolution 1.76Å | |PDB= 1ht9 |SIZE=350|CAPTION= <scene name='initialview01'>1ht9</scene>, resolution 1.76Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ht9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ht9 OCA], [http://www.ebi.ac.uk/pdbsum/1ht9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ht9 RCSB]</span> | ||
}} | }} | ||
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[[Category: Linse, S.]] | [[Category: Linse, S.]] | ||
[[Category: Svensson, A L.]] | [[Category: Svensson, A L.]] | ||
| - | [[Category: CA]] | ||
[[Category: calbindin d9k]] | [[Category: calbindin d9k]] | ||
[[Category: calcium binding]] | [[Category: calcium binding]] | ||
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[[Category: folding]] | [[Category: folding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:10:06 2008'' |
Revision as of 18:10, 30 March 2008
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| , resolution 1.76Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DOMAIN SWAPPING EF-HANDS
Overview
The structure of calbindin D(9k) with two substitutions was determined by X-ray crystallography at 1.8-A resolution. Unlike wild-type calbindin D(9k), which is a monomeric protein with two EF-hands, the structure of the mutated calbindin D(9k) reveals an intertwined dimer. In the dimer, two EF-hands of the monomers have exchanged places, and thus a 3D domain-swapped dimer has been formed. EF-hand I of molecule A is packed toward EF-hand II of molecule B and vice versa. The formation of a hydrophobic cluster, in a region linking the EF-hands, promotes the conversion of monomers to 3D domain-swapped dimers. We propose a mechanism by which domain swapping takes place via the apo form of calbindin D(9k). Once formed, the calbindin D(9k) dimers are remarkably stable, as with even larger misfolded aggregates like amyloids. Thus calbindin D(9k) dimers cannot be converted to monomers by dilution. However, heating can be used for conversion, indicating high energy barriers separating monomers from dimers.
About this Structure
1HT9 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
An extended hydrophobic core induces EF-hand swapping., Hakansson M, Svensson A, Fast J, Linse S, Protein Sci. 2001 May;10(5):927-33. PMID:11316872
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