1hv6
From Proteopedia
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|PDB= 1hv6 |SIZE=350|CAPTION= <scene name='initialview01'>1hv6</scene>, resolution 2.00Å | |PDB= 1hv6 |SIZE=350|CAPTION= <scene name='initialview01'>1hv6</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=GCU:D-GLUCURONIC+ACID'>GCU</scene>, <scene name='pdbligand=MAV:D-MANNURONIC+ACID'>MAV</scene>, <scene name='pdbligand=MAW:4-DEOXY-D-MANNURONIC+ACID'>MAW</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Poly(beta-D-mannuronate)_lyase Poly(beta-D-mannuronate) lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.3 4.2.2.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Poly(beta-D-mannuronate)_lyase Poly(beta-D-mannuronate) lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.2.3 4.2.2.3] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1qaz|1QAZ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hv6 OCA], [http://www.ebi.ac.uk/pdbsum/1hv6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hv6 RCSB]</span> | ||
}} | }} | ||
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[[Category: Murata, K.]] | [[Category: Murata, K.]] | ||
[[Category: Yoon, H J.]] | [[Category: Yoon, H J.]] | ||
| - | [[Category: SO4]] | ||
[[Category: alginate lyase]] | [[Category: alginate lyase]] | ||
| - | + | [[Category: trisaccharide complex,alpha barrel]] | |
| - | [[Category: trisaccharide complex]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:10:50 2008'' |
Revision as of 18:10, 30 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Activity: | Poly(beta-D-mannuronate) lyase, with EC number 4.2.2.3 | ||||||
| Related: | 1QAZ
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III COMPLEXED WITH TRISACCHARIDE PRODUCT.
Overview
The structure of A1-III from a Sphingomonas species A1 complexed with a trisaccharide product (4-deoxy-l-erythro-hex-4-enepyranosyluronate-mannuronate-mannuronic acid) was determined by X-ray crystallography at 2.0 A with an R-factor of 0.16. The final model of the complex form comprising 351 amino acid residues, 245 water molecules, one sulfate ion and one trisaccharide product exhibited a C(alpha) r.m.s.d. value of 0.154 A with the reported apo form of the enzyme. The trisaccharide was bound in the active cleft at subsites -3 approximately -1 from the non-reducing end by forming several hydrogen bonds and van der Waals interactions with protein atoms. The catalytic residue was estimated to be Tyr246, which existed between subsites -1 and +1 based on a mannuronic acid model oriented at subsite +1.
About this Structure
1HV6 is a Single protein structure of sequence from Sphingomonas species. Full crystallographic information is available from OCA.
Reference
Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 A resolution., Yoon HJ, Hashimoto W, Miyake O, Murata K, Mikami B, J Mol Biol. 2001 Mar 16;307(1):9-16. PMID:11243798
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