5hoq
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Apo structure of CalS11, TDP-rhamnose 3'-o-methyltransferase, an enzyme in Calicheamicin biosynthesis== | |
| + | <StructureSection load='5hoq' size='340' side='right' caption='[[5hoq]], [[Resolution|resolution]] 1.79Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5hoq]] is a 5 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4pwr 4pwr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HOQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HOQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hoq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hoq OCA], [http://pdbe.org/5hoq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hoq RCSB], [http://www.ebi.ac.uk/pdbsum/5hoq PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Structure analysis and ensemble refinement of the apo-structure of thymidine diphosphate (TDP)-rhamnose 3'-O-methyltransferase reveal a gate for substrate entry and product release. TDP-rhamnose 3'-O-methyltransferase (CalS11) catalyses a 3'-O-methylation of TDP-rhamnose, an intermediate in the biosynthesis of enediyne antitumor antibiotic calicheamicin. CalS11 operates at the sugar nucleotide stage prior to glycosylation step. Here, we present the crystal structure of the apo form of CalS11 at 1.89 A resolution. We propose that the L2 loop functions as a gate facilitating and/or providing specificity for substrate entry or promoting product release. Ensemble refinement analysis slightly improves the crystallographic refinement statistics and furthermore provides a compelling way to visualize the dynamic model of loop L2, supporting the understanding of its proposed role in catalysis. | ||
| - | + | Loop dynamics of thymidine diphosphate-rhamnose 3'-O-methyltransferase (CalS11), an enzyme in calicheamicin biosynthesis.,Han L, Singh S, Thorson JS, Phillips GN Jr Struct Dyn. 2016 Feb 18;3(1):012004. doi: 10.1063/1.4941368. eCollection 2016, Jan. PMID:26958582<ref>PMID:26958582</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 5hoq" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bingman, C A]] | ||
| + | [[Category: Biosynthesis, Enzyme Discovery for Natural Product]] | ||
[[Category: Han, L]] | [[Category: Han, L]] | ||
| - | [[Category: | + | [[Category: Helmich, K E]] |
| + | [[Category: Phillips, G N]] | ||
[[Category: Singh, S]] | [[Category: Singh, S]] | ||
| - | [[Category: | + | [[Category: Thorson, J S]] |
| - | [[Category: | + | [[Category: Rhamnose methyltransferase calicheamicin biosynthesis]] |
| - | [[Category: | + | [[Category: Transferase]] |
Revision as of 03:23, 11 May 2016
Apo structure of CalS11, TDP-rhamnose 3'-o-methyltransferase, an enzyme in Calicheamicin biosynthesis
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