1i6h
From Proteopedia
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|PDB= 1i6h |SIZE=350|CAPTION= <scene name='initialview01'>1i6h</scene>, resolution 3.30Å | |PDB= 1i6h |SIZE=350|CAPTION= <scene name='initialview01'>1i6h</scene>, resolution 3.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1i3q|1I3Q]], [[1i50|1I50]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i6h OCA], [http://www.ebi.ac.uk/pdbsum/1i6h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i6h RCSB]</span> | ||
}} | }} | ||
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[[Category: Gnatt, A L.]] | [[Category: Gnatt, A L.]] | ||
[[Category: Kornberg, R D.]] | [[Category: Kornberg, R D.]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: ZN]] | ||
[[Category: dna]] | [[Category: dna]] | ||
[[Category: molecular machine]] | [[Category: molecular machine]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:15:15 2008'' |
Revision as of 18:15, 30 March 2008
| |||||||
| , resolution 3.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , , , | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Related: | 1I3Q, 1I50
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RNA POLYMERASE II ELONGATION COMPLEX
Overview
The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 A resolution. Duplex DNA is seen entering the main cleft of the enzyme and unwinding before the active site. Nine base pairs of DNA-RNA hybrid extend from the active center at nearly right angles to the entering DNA, with the 3' end of the RNA in the nucleotide addition site. The 3' end is positioned above a pore, through which nucleotides may enter and through which RNA may be extruded during back-tracking. The 5'-most residue of the RNA is close to the point of entry to an exit groove. Changes in protein structure between the transcribing complex and free enzyme include closure of a clamp over the DNA and RNA and ordering of a series of "switches" at the base of the clamp to create a binding site complementary to the DNA-RNA hybrid. Protein-nucleic acid contacts help explain DNA and RNA strand separation, the specificity of RNA synthesis, "abortive cycling" during transcription initiation, and RNA and DNA translocation during transcription elongation.
About this Structure
1I6H is a Protein complex structure of sequences from Saccharomyces cerevisiae. The following page contains interesting information on the relation of 1I6H with [RNA Polymerase]. Full crystallographic information is available from OCA.
Reference
Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution., Gnatt AL, Cramer P, Fu J, Bushnell DA, Kornberg RD, Science. 2001 Jun 8;292(5523):1876-82. Epub 2001 Apr 19. PMID:11313499
Page seeded by OCA on Sun Mar 30 21:15:15 2008
