1i6v
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1i6v |SIZE=350|CAPTION= <scene name='initialview01'>1i6v</scene>, resolution 3.3Å | |PDB= 1i6v |SIZE=350|CAPTION= <scene name='initialview01'>1i6v</scene>, resolution 3.3Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=RFP:RIFAMPICIN'>RFP</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1hqm|1HQM]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i6v OCA], [http://www.ebi.ac.uk/pdbsum/1i6v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i6v RCSB]</span> | ||
}} | }} | ||
| Line 29: | Line 32: | ||
[[Category: Murakami, K.]] | [[Category: Murakami, K.]] | ||
[[Category: Mustaev, A.]] | [[Category: Mustaev, A.]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: RFP]] | ||
| - | [[Category: ZN]] | ||
[[Category: 3d- structure]] | [[Category: 3d- structure]] | ||
[[Category: dna-directed rna polymerase]] | [[Category: dna-directed rna polymerase]] | ||
| Line 37: | Line 37: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:15:24 2008'' |
Revision as of 18:15, 30 March 2008
| |||||||
| , resolution 3.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Related: | 1HQM
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THERMUS AQUATICUS CORE RNA POLYMERASE-RIFAMPICIN COMPLEX
Overview
Rifampicin (Rif) is one of the most potent and broad spectrum antibiotics against bacterial pathogens and is a key component of anti-tuberculosis therapy, stemming from its inhibition of the bacterial RNA polymerase (RNAP). We determined the crystal structure of Thermus aquaticus core RNAP complexed with Rif. The inhibitor binds in a pocket of the RNAP beta subunit deep within the DNA/RNA channel, but more than 12 A away from the active site. The structure, combined with biochemical results, explains the effects of Rif on RNAP function and indicates that the inhibitor acts by directly blocking the path of the elongating RNA when the transcript becomes 2 to 3 nt in length.
About this Structure
1I6V is a Protein complex structure of sequences from Thermus aquaticus. Full crystallographic information is available from OCA.
Reference
Structural mechanism for rifampicin inhibition of bacterial rna polymerase., Campbell EA, Korzheva N, Mustaev A, Murakami K, Nair S, Goldfarb A, Darst SA, Cell. 2001 Mar 23;104(6):901-12. PMID:11290327
Page seeded by OCA on Sun Mar 30 21:15:24 2008
