1i9z
From Proteopedia
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|PDB= 1i9z |SIZE=350|CAPTION= <scene name='initialview01'>1i9z</scene>, resolution 1.8Å | |PDB= 1i9z |SIZE=350|CAPTION= <scene name='initialview01'>1i9z</scene>, resolution 1.8Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=2IP:D-MYO-INOSITOL-1,4-BISPHOSPHATE'>2IP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1i9y|1I9Y]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i9z OCA], [http://www.ebi.ac.uk/pdbsum/1i9z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1i9z RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Tsujishita, Y.]] | [[Category: Tsujishita, Y.]] | ||
[[Category: York, J D.]] | [[Category: York, J D.]] | ||
| - | + | [[Category: ip2,]] | |
| - | + | ||
| - | [[Category: ip2]] | + | |
[[Category: ip3]] | [[Category: ip3]] | ||
[[Category: ipp5c]] | [[Category: ipp5c]] | ||
[[Category: spsynaptojanin]] | [[Category: spsynaptojanin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:16:46 2008'' |
Revision as of 18:16, 30 March 2008
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| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1I9Y
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN IN COMPLEX WITH INOSITOL (1,4)-BISPHOSPHATE AND CALCIUM ION
Overview
Inositol polyphosphate 5-phosphatases are central to intracellular processes ranging from membrane trafficking to Ca(2+) signaling, and defects in this activity result in the human disease Lowe syndrome. The 1.8 resolution structure of the inositol polyphosphate 5-phosphatase domain of SPsynaptojanin bound to Ca(2+) and inositol (1,4)-bisphosphate reveals a fold and an active site His and Asp pair resembling those of several Mg(2+)-dependent nucleases. Additional loops mediate specific inositol polyphosphate contacts. The 4-phosphate of inositol (1,4)-bisphosphate is misoriented by 4.6 compared to the reactive geometry observed in the apurinic/apyrimidinic endonuclease 1, explaining the dephosphorylation site selectivity of the 5-phosphatases. Based on the structure, a series of mutants are described that exhibit altered substrate specificity providing general determinants for substrate recognition.
About this Structure
1I9Z is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.
Reference
Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase., Tsujishita Y, Guo S, Stolz LE, York JD, Hurley JH, Cell. 2001 May 4;105(3):379-89. PMID:11348594
Page seeded by OCA on Sun Mar 30 21:16:46 2008
Categories: Schizosaccharomyces pombe | Single protein | Guo, S. | Hurley, J H. | Stolz, L. | Tsujishita, Y. | York, J D. | Ip2, | Ip3 | Ipp5c | Spsynaptojanin
