5daj
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of NalD, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa== | |
| + | <StructureSection load='5daj' size='340' side='right' caption='[[5daj]], [[Resolution|resolution]] 2.65Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5daj]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DAJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5daj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5daj OCA], [http://pdbe.org/5daj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5daj RCSB], [http://www.ebi.ac.uk/pdbsum/5daj PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | NalD was reported to be the secondary repressor of the MexAB-OprM multidrug efflux pump, the major system contributing to intrinsic multidrug resistance in Pseudomonas aeruginosa. Here, we show that novobiocin binds directly to NalD, which leads NalD to dissociate from the DNA promoter, and thus de-represses the expression of the MexAB-OprM pump. In addition, we have solved the crystal structure of NalD at a resolution of 2.90 A. The structural alignment of NalD to its homologue TtgR reveals that the residues N129 and H167 in NalD are involved in its novobiocin-binding ability. We have confirmed the function of these two amino acids by EMSA and plate assay. The results presented here highlight the importance and diversity of regulatory mechanism in bacterial antibiotic resistance, and provide further insight for novel antimicrobial development. This article is protected by copyright. All rights reserved. | ||
| - | + | Novobiocin binding to NalD induces the expression of the MexAB-OprM pump in Pseudomonas aeruginosa.,Chen W, Wang D, Zhou W, Sang H, Liu X, Ge Z, Zhang J, Lan L, Yang CG, Chen H Mol Microbiol. 2016 Feb 4. doi: 10.1111/mmi.13346. PMID:26844397<ref>PMID:26844397</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5daj" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Chen, H]] | [[Category: Chen, H]] | ||
| - | [[Category: | + | [[Category: Chen, W Z]] |
| - | [[Category: Gan, J | + | [[Category: Gan, J H]] |
| - | [[Category: | + | [[Category: Hu, Q Y]] |
| - | [[Category: Huang, S | + | [[Category: Huang, S Q]] |
| + | [[Category: Liu, X C]] | ||
| + | [[Category: Wang, D]] | ||
| + | [[Category: Repressor]] | ||
| + | [[Category: Tetr family]] | ||
| + | [[Category: Transcription regulator]] | ||
| + | [[Category: Transcriptional regulator]] | ||
Revision as of 18:42, 12 May 2016
Crystal structure of NalD, the secondary repressor of MexAB-OprM multidrug efflux pump in Pseudomonas aeruginosa
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