1ig9
From Proteopedia
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|PDB= 1ig9 |SIZE=350|CAPTION= <scene name='initialview01'>1ig9</scene>, resolution 2.60Å | |PDB= 1ig9 |SIZE=350|CAPTION= <scene name='initialview01'>1ig9</scene>, resolution 2.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DOC:2',3'-DIDEOXYCYTIDINE-5'-MONOPHOSPHATE'>DOC</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=TTP:THYMIDINE-5'-TRIPHOSPHATE'>TTP</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span> |
| - | |GENE= gp43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= gp43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12353 Enterobacteria phage RB69]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1waj|1WAJ]], [[1clq|1CLQ]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ig9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ig9 OCA], [http://www.ebi.ac.uk/pdbsum/1ig9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ig9 RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1IG9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1IG9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb69 Enterobacteria phage rb69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IG9 OCA]. |
==Reference== | ==Reference== | ||
Structure of the replicating complex of a pol alpha family DNA polymerase., Franklin MC, Wang J, Steitz TA, Cell. 2001 Jun 1;105(5):657-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11389835 11389835] | Structure of the replicating complex of a pol alpha family DNA polymerase., Franklin MC, Wang J, Steitz TA, Cell. 2001 Jun 1;105(5):657-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11389835 11389835] | ||
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
| - | [[Category: Enterobacteria phage | + | [[Category: Enterobacteria phage rb69]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Franklin, M C.]] | [[Category: Franklin, M C.]] | ||
[[Category: Steitz, T A.]] | [[Category: Steitz, T A.]] | ||
[[Category: Wang, J.]] | [[Category: Wang, J.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: TTP]] | ||
[[Category: catalytic complex]] | [[Category: catalytic complex]] | ||
[[Category: dna polymerase]] | [[Category: dna polymerase]] | ||
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[[Category: two metal ion mechanism]] | [[Category: two metal ion mechanism]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:19:22 2008'' |
Revision as of 18:19, 30 March 2008
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| , resolution 2.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||
| Gene: | gp43 (Enterobacteria phage RB69) | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Related: | 1WAJ, 1CLQ
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the Replicating Complex of a Pol Alpha Family DNA Polymerase
Overview
We describe the 2.6 A resolution crystal structure of RB69 DNA polymerase with primer-template DNA and dTTP, capturing the step just before primer extension. This ternary complex structure in the human DNA polymerase alpha family shows a 60 degrees rotation of the fingers domain relative to the apo-protein structure, similar to the fingers movement in pol I family polymerases. Minor groove interactions near the primer 3' terminus suggest a common fidelity mechanism for pol I and pol alpha family polymerases. The duplex product DNA orientation differs by 40 degrees between the polymerizing mode and editing mode structures. The role of the thumb in this DNA motion provides a model for editing in the pol alpha family.
About this Structure
1IG9 is a Single protein structure of sequence from Enterobacteria phage rb69. Full crystallographic information is available from OCA.
Reference
Structure of the replicating complex of a pol alpha family DNA polymerase., Franklin MC, Wang J, Steitz TA, Cell. 2001 Jun 1;105(5):657-67. PMID:11389835
Page seeded by OCA on Sun Mar 30 21:19:22 2008
