Dicer
From Proteopedia
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The Human Dicer and its mechanism is of the utmost importance because it cleaves these dsRNA's to form small interfering RNA or microRNA, which are then integrated into the RNA-induced silencing complex, or the RISC complex.(2,4) This complex then targets mRNA and prevents translation by disrupting the targeted gene. Without Dicer, gene silencing cannot occur. Therefore, without Dicer, DNA and RNA cannot be regulated. | The Human Dicer and its mechanism is of the utmost importance because it cleaves these dsRNA's to form small interfering RNA or microRNA, which are then integrated into the RNA-induced silencing complex, or the RISC complex.(2,4) This complex then targets mRNA and prevents translation by disrupting the targeted gene. Without Dicer, gene silencing cannot occur. Therefore, without Dicer, DNA and RNA cannot be regulated. | ||
| - | == | + | ==Introduction== |
| - | Dicer is known to be a direct cause of macular degeneration. The abscence of Dicer in retinal pigment epithelium causes the eye to break down into macular degeneration. It is hypothesized that Dicer has a specific role in maintaining this retinal health.(5) | ||
| - | In cancer cells, especially in lung and ovarian cancers, low dicer levels allow for malignant cells to duplicate and develop. In prostate and esophageal cancers, high dicer counts are a direct correlation to poor patient prognosis. Dicer's role is considered to be unique in different cancer types because of these relations.(5) | ||
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| - | ==Introduction== | ||
Dicer is a type of [[Ribonuclease]] that processes potentially harmful double-stranded RNA (dsRNA) into microRNA and small-interfering RNA (siRNA) to be used in the process of RNA interference. Dicer is commonly utilized by cells in order to prevent the assimilation of viral DNA into the cells’ genome. The viral DNA is butchered into smaller segments that are each about 21 nucleotides long; the cut take places at the 5’ phosphate and the 3’ hydroxyl, and usually includes a 2 nucleotide overhang. There is a single processing center in HS Dicer implying that there are two catalytic sites which help form products with the 2 3' overhang. These newly formed segments attach themselves to single stranded mRNA which ultimately leads to mRNA degradation by the cell and translational suppression. The dicer enzyme in humans contains three domains: the <scene name='70/706244/Rnase_iii_1/1'>RNase III 1</scene>, <scene name='70/706244/Rnase_iii_2/1'>RNase III 2</scene>, and the <scene name='70/706244/Paz_domain/1'>Paz Domain</scene>.<ref>PMID: 16410517</ref> There are three classes of RNase III proteins which are divided into categories called Escherichia coli RNase III, <scene name='70/706244/Drosha/1'>Drosha</scene>, and Dicer which are given the numbers one, two, and three respectively. The Escherichia coli RNase III class has one domain while the Drosha and dicer have two domains each. There is no evidence of the first class of enzymes in mammals. | Dicer is a type of [[Ribonuclease]] that processes potentially harmful double-stranded RNA (dsRNA) into microRNA and small-interfering RNA (siRNA) to be used in the process of RNA interference. Dicer is commonly utilized by cells in order to prevent the assimilation of viral DNA into the cells’ genome. The viral DNA is butchered into smaller segments that are each about 21 nucleotides long; the cut take places at the 5’ phosphate and the 3’ hydroxyl, and usually includes a 2 nucleotide overhang. There is a single processing center in HS Dicer implying that there are two catalytic sites which help form products with the 2 3' overhang. These newly formed segments attach themselves to single stranded mRNA which ultimately leads to mRNA degradation by the cell and translational suppression. The dicer enzyme in humans contains three domains: the <scene name='70/706244/Rnase_iii_1/1'>RNase III 1</scene>, <scene name='70/706244/Rnase_iii_2/1'>RNase III 2</scene>, and the <scene name='70/706244/Paz_domain/1'>Paz Domain</scene>.<ref>PMID: 16410517</ref> There are three classes of RNase III proteins which are divided into categories called Escherichia coli RNase III, <scene name='70/706244/Drosha/1'>Drosha</scene>, and Dicer which are given the numbers one, two, and three respectively. The Escherichia coli RNase III class has one domain while the Drosha and dicer have two domains each. There is no evidence of the first class of enzymes in mammals. | ||
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==Pathology== | ==Pathology== | ||
Mutations involving the dicer protein have been linked to the development of diseases in humans. Conditions such as pleuropulmonary blastoma<ref>PMID: 19556464</ref>, goiter multinodular<ref>PMID: 21205968</ref>, and rhabdomyosarcoma<ref>PMID: 21882293</ref> are related to dicer malfunction. Pleuropulmonary blastoma, goiter multinodular, cystic nephroma, and Sertoli-Leydig cell tumors are due a mutation in the Dicer1 gene given the name Dicer1 Syndrome. Dicer1 Syndrome is an inherited disorder that causes the risk of malignant tumors and benign tumors to increase. This occurs because short Dicer proteins are formed that cannot help in the production of miRNA, which can cause cells to grow into tumors. The risk of tumors is mainly increased in the lungs, kidneys, ovaries, and thyroid. Dicer1 Syndrome is transferred in an autosomal dominant pattern. The top treatment is surgery to remove the tumor. | Mutations involving the dicer protein have been linked to the development of diseases in humans. Conditions such as pleuropulmonary blastoma<ref>PMID: 19556464</ref>, goiter multinodular<ref>PMID: 21205968</ref>, and rhabdomyosarcoma<ref>PMID: 21882293</ref> are related to dicer malfunction. Pleuropulmonary blastoma, goiter multinodular, cystic nephroma, and Sertoli-Leydig cell tumors are due a mutation in the Dicer1 gene given the name Dicer1 Syndrome. Dicer1 Syndrome is an inherited disorder that causes the risk of malignant tumors and benign tumors to increase. This occurs because short Dicer proteins are formed that cannot help in the production of miRNA, which can cause cells to grow into tumors. The risk of tumors is mainly increased in the lungs, kidneys, ovaries, and thyroid. Dicer1 Syndrome is transferred in an autosomal dominant pattern. The top treatment is surgery to remove the tumor. | ||
| + | Dicer is known to be a direct cause of macular degeneration. The abscence of Dicer in retinal pigment epithelium causes the eye to break down into macular degeneration. It is hypothesized that Dicer has a specific role in maintaining this retinal health.(5) | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 17:27, 23 May 2016
Dicer
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Proteopedia Page Contributors and Editors (what is this?)
Justin Woodard, Sam Hayes, Michal Harel, Ann Taylor, Wally Novak, Alexander Berchansky
