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<scene name='58/583419/Pka_reg/1'>The PKA catalytic unit crystalized with its regulatory unit</scene>, pdb structure [[3fhi]]<ref>PMID:15692043</ref>, is shown with the catalytic unit's small lobe in ''gray'' and large lobe in ''tan'', and the regulatory unit in ''light blue''.
<scene name='58/583419/Pka_reg/1'>The PKA catalytic unit crystalized with its regulatory unit</scene>, pdb structure [[3fhi]]<ref>PMID:15692043</ref>, is shown with the catalytic unit's small lobe in ''gray'' and large lobe in ''tan'', and the regulatory unit in ''light blue''.
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There is <scene name='58/583419/Pka_reg/4'>significant distance from the regulatory unit's residues to the residues from exon 1 of the catalytic unit</scene>. Here the residues which interact with the regulatory unit '''R''' are represent with halos and ball-and-stick. The residues from the catalytic unit's ''exon 1'' are labelled by residue index. Note that the last three residues from exon 1 (residues # 13-15) are the only amino acids from the first exon of the catalytic unit that were included in this crystal structure.
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The <scene name='58/583419/Pka_reg/4'>residues from exon 1 of the catalytic unit do not directly interact with the regulatory unit</scene>. Here the catalytic unit's residues which do interact with the regulatory unit are represent with halos and ball-and-stick. The residues from the catalytic unit's ''exon 1'' are labelled by residue index. Note that the last three residues from exon 1 (residues # 13-15) are the only amino acids from the first exon of the catalytic unit that were included in this crystal structure. As seen in the structure, the exon 1 residues are over 30 angstroms from the regulatory unit - too far to bind.
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These three residues are also <scene name='58/583419/Pka_reg/3'>distant from PKI-interacting residues (halos and ball and stick)</scene> - a second regulator/inhibitor of 'C', as well.
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These three residues are also <scene name='58/583419/Pka_reg/3'>distant from PKI-interacting residues (halos and ball and stick)</scene> - a second regulator/inhibitor of the catalytic unit, as well.
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This is also obvious from the pdb structure for the <scene name='58/583419/Pka_pki/1'>crystal of C bound to PKI (purple)</scene>, [[1atp]]<ref>PMID:15299527</ref>.
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This is also obvious from the pdb structure for the <scene name='58/583419/Pka_pki/1'>crystal of the catalytic unit bound to PKI (purple)</scene>, [[1atp]]<ref>PMID:15299527</ref>.
<scene name='58/583419/Pka_dimer/3'>showing residues 1-15 with halos</scene>
<scene name='58/583419/Pka_dimer/3'>showing residues 1-15 with halos</scene>

Revision as of 17:21, 29 May 2016

PKA and Regulators

Caption for this structure

Drag the structure with the mouse to rotate

References

  1. ↑ Kim C, Xuong NH, Taylor SS. Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA. Science. 2005 Feb 4;307(5710):690-6. PMID:15692043 doi:307/5710/690
  2. ↑ Zheng J, Trafny EA, Knighton DR, Xuong NH, Taylor SS, Ten Eyck LF, Sowadski JM. 2.2 A refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MnATP and a peptide inhibitor. Acta Crystallogr D Biol Crystallogr. 1993 May 1;49(Pt 3):362-5. PMID:15299527 doi:10.1107/S0907444993000423

Proteopedia Page Contributors and Editors (what is this?)

Joseph M. Steinberger

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