JMS/Sandbox21
From Proteopedia
(Difference between revisions)
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The <scene name='58/583419/Pka_reg/4'>residues from exon 1 of the catalytic unit do not directly interact with the regulatory unit</scene>. Here the catalytic unit's residues which do interact with the regulatory unit are represent with halos and ball-and-stick. The residues from exon 1 of the catalytic unit are labelled by residue index. Note that the last three residues from exon 1 (residues # 13-15) are the only amino acids from the first exon of the catalytic unit that were included in this crystal structure. As seen in the structure, the exon 1 residues are over 30 angstroms from the regulatory unit - too far to bind. | The <scene name='58/583419/Pka_reg/4'>residues from exon 1 of the catalytic unit do not directly interact with the regulatory unit</scene>. Here the catalytic unit's residues which do interact with the regulatory unit are represent with halos and ball-and-stick. The residues from exon 1 of the catalytic unit are labelled by residue index. Note that the last three residues from exon 1 (residues # 13-15) are the only amino acids from the first exon of the catalytic unit that were included in this crystal structure. As seen in the structure, the exon 1 residues are over 30 angstroms from the regulatory unit - too far to bind. | ||
| - | The crystal structure for the tetromeric holoenzyme, [[3tnp]], supports the above observation. There is no interaction between <scene name='58/583419/Pka_dimer/3'>exon 1 residues</scene>, shown with halos, to the regulatory | + | The crystal structure for the tetromeric holoenzyme, [[3tnp]], supports the above observation. There is no interaction between <scene name='58/583419/Pka_dimer/3'>exon 1 residues</scene>, shown with halos, to the regulatory units, shown in green and purple. |
These three residues are also <scene name='58/583419/Pka_reg/3'>distant from PKI-interacting residues (halos and ball and stick)</scene> - a second regulator/inhibitor of the catalytic unit, as well. | These three residues are also <scene name='58/583419/Pka_reg/3'>distant from PKI-interacting residues (halos and ball and stick)</scene> - a second regulator/inhibitor of the catalytic unit, as well. | ||
Revision as of 17:30, 29 May 2016
PKA and Regulators
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References
- ↑ Kim C, Xuong NH, Taylor SS. Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA. Science. 2005 Feb 4;307(5710):690-6. PMID:15692043 doi:307/5710/690
- ↑ Zheng J, Trafny EA, Knighton DR, Xuong NH, Taylor SS, Ten Eyck LF, Sowadski JM. 2.2 A refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MnATP and a peptide inhibitor. Acta Crystallogr D Biol Crystallogr. 1993 May 1;49(Pt 3):362-5. PMID:15299527 doi:10.1107/S0907444993000423
