1io9

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|PDB= 1io9 |SIZE=350|CAPTION= <scene name='initialview01'>1io9</scene>, resolution 2.05&Aring;
|PDB= 1io9 |SIZE=350|CAPTION= <scene name='initialview01'>1io9</scene>, resolution 2.05&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
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|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=[[1io7|1IO7]], [[1io8|1IO8]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1io9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1io9 OCA], [http://www.ebi.ac.uk/pdbsum/1io9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1io9 RCSB]</span>
}}
}}
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[[Category: Sligar, S G.]]
[[Category: Sligar, S G.]]
[[Category: Yamane, K.]]
[[Category: Yamane, K.]]
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[[Category: HEM]]
 
[[Category: crystal structure]]
[[Category: crystal structure]]
[[Category: cytochromo p450]]
[[Category: cytochromo p450]]
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[[Category: thermophilic]]
[[Category: thermophilic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:52:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:22:24 2008''

Revision as of 18:22, 30 March 2008

Image:1io9.jpg


PDB ID 1io9

Drag the structure with the mouse to rotate
, resolution 2.05Å
Ligands:
Related: 1IO7, 1IO8


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES


Overview

Crystal structures of a thermostable cytochrome P450 (CYP119) and a site-directed mutant, (Phe24Leu), from the acidothermophilic archaea Sulfolobus solfataricus were determined at 1.5-2.0 A resolution. We identify important crystallographic waters in the ferric heme pocket, observe protein conformational changes upon inhibitor binding, and detect a unique distribution of surface charge not found in other P450s. An analysis of factors contributing to thermostability of CYP119 of these high resolution structures shows an apparent increase in clustering of aromatic residues and optimum stacking. The contribution of aromatic stacking was investigated further with the mutant crystal structure and differential scanning calorimetry.

About this Structure

1IO9 is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Thermophilic cytochrome P450 (CYP119) from Sulfolobus solfataricus: high resolution structure and functional properties., Park SY, Yamane K, Adachi S, Shiro Y, Weiss KE, Maves SA, Sligar SG, J Inorg Biochem. 2002 Sep 20;91(4):491-501. PMID:12237217

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