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5i8f
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of St. John's wort Hyp-1 protein in complex with melatonin== | |
| + | <StructureSection load='5i8f' size='340' side='right' caption='[[5i8f]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5i8f]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I8F FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ML1:N-[2-(5-METHOXY-1H-INDOL-3-YL)ETHYL]ACETAMIDE'>ML1</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i8f OCA], [http://pdbe.org/5i8f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i8f RCSB], [http://www.ebi.ac.uk/pdbsum/5i8f PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hypericin, a red-colored naphtodianthrone, is a natural product synthesized in the medicinal plant Hypericum perforatum, widely known as St. John's wort. Hypericin has been attracting a growing attention of the pharmaceutical industry because of its potential application in various therapies, including the treatment of depression. In vivo, hypericin is synthesized by dimerization of emodin in a complicated multistep reaction that is reportedly catalyzed by a small (17.8kDa) protein, Hyp-1. Based on relatively low sequence similarity ( approximately 50%), Hyp-1 has been tentatively classified as a plant PR-10 (pathogenesis-related class 10) protein. Members of the PR-10 family are ubiquitous plant proteins associated with stress control and tissue differentiation but with no clearly understood molecular mechanism. They have, however, a well-defined folding canon, consisting of an extended antiparallel beta-sheet wrapped around a C-terminal alpha-helix, enclosing in the protein interior a huge cavity, in which various hydrophobic ligands can be bound. Apart from Hyp-1, only two other PR-10 members have been found to possess enzymatic activity (S-norcoclaurine synthase and TcmN aromatase/cyclase). In this paper, we report a high-resolution crystal structure of Hyp-1, confirming that it indeed has a PR-10 fold. The protein binds multiple polyethylene glycol molecules, some of which occupy the hydrophobic cavity. The crystallographic model illustrates a high degree of conformational adaptability of both interacting partners for efficient binding. We have been unable, however, to dimerize emodin to hypericin using Hyp-1 as biocatalyst. This puzzling result does not have a clear explanation at this time. | ||
| - | + | Crystal structure of Hyp-1, a St. John's wort protein implicated in the biosynthesis of hypericin.,Michalska K, Fernandes H, Sikorski M, Jaskolski M J Struct Biol. 2010 Feb;169(2):161-71. Epub 2009 Oct 21. PMID:19853038<ref>PMID:19853038</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 5i8f" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Dauter, Z]] | [[Category: Dauter, Z]] | ||
| - | [[Category: Sliwiak, J]] | ||
[[Category: Jaskolski, M]] | [[Category: Jaskolski, M]] | ||
| + | [[Category: Sliwiak, J]] | ||
| + | [[Category: Cytokinin]] | ||
| + | [[Category: Depression]] | ||
| + | [[Category: Hydrophobic cavity]] | ||
| + | [[Category: Hypericin]] | ||
| + | [[Category: Melatonin]] | ||
| + | [[Category: Pathogenesis-related protein]] | ||
| + | [[Category: Phytohormone binding]] | ||
| + | [[Category: Plant defense]] | ||
| + | [[Category: Plant hormone binding]] | ||
| + | [[Category: Plant protein]] | ||
| + | [[Category: Pr-10 fold]] | ||
| + | [[Category: Pr-10 protein]] | ||
Revision as of 23:00, 1 June 2016
Crystal structure of St. John's wort Hyp-1 protein in complex with melatonin
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