5i2g
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i2g OCA], [http://pdbe.org/5i2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i2g RCSB], [http://www.ebi.ac.uk/pdbsum/5i2g PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i2g OCA], [http://pdbe.org/5i2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i2g RCSB], [http://www.ebi.ac.uk/pdbsum/5i2g PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glycyl radical enzymes (GREs) represent a diverse superfamily of enzymes that utilize a radical mechanism to catalyze difficult, but often essential, chemical reactions. In this work, we present the first biochemical and structural data for a GRE-type diol dehydratase from the organism Roseburia inulinivorans (RiDD). Despite high sequence (48% identity) and structural similarity to the GRE-type glycerol dehydratase from Clostridium butyricum (CbGD), we demonstrate that the RiDD is in fact a diol dehydratase. In addition, the RiDD will utilize both (S)-1,2-propanediol and (R)-1,2-propanediol as a substrate, with an observed preference for the (S) enantiomer. Based on the new structural information we develop and successfully test a hypothesis that explains the functional differences we observe. | ||
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| + | 1,2-propanediol Dehydration in Roseburia inulinivorans; Structural Basis for Substrate and Enantiomer Selectivity.,LaMattina JW, Keul ND, Reitzer P, Kapoor S, Galzerani F, Koch DJ, Gouvea IE, Lanzilotta WN J Biol Chem. 2016 Jun 1. pii: jbc.M116.721142. PMID:27252380<ref>PMID:27252380</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5i2g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
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[[Category: Koch, D J]] | [[Category: Koch, D J]] | ||
[[Category: LaMattina, J W]] | [[Category: LaMattina, J W]] | ||
| + | [[Category: Lanzilotta, W N]] | ||
[[Category: Reitzer, P]] | [[Category: Reitzer, P]] | ||
[[Category: Diol dehydratase]] | [[Category: Diol dehydratase]] | ||
[[Category: Enzyme structure]] | [[Category: Enzyme structure]] | ||
| - | [[Category: Glycerol dehydratase]] | ||
[[Category: Glycyl radical enzyme]] | [[Category: Glycyl radical enzyme]] | ||
[[Category: Lyase]] | [[Category: Lyase]] | ||
Revision as of 07:45, 20 June 2016
1,2-propanediol Dehydration in Roseburia inulinivorans; Structural Basis for Substrate and Enantiomer Selectivity
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