1j81
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1j81 |SIZE=350|CAPTION= <scene name='initialview01'>1j81</scene>, resolution 2.20Å | |PDB= 1j81 |SIZE=350|CAPTION= <scene name='initialview01'>1j81</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[ij7z|IJ7Z]], [[1j80|1J80]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j81 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j81 OCA], [http://www.ebi.ac.uk/pdbsum/1j81 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j81 RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Ratnaparkhi, G S.]] | [[Category: Ratnaparkhi, G S.]] | ||
[[Category: Varadarajan, R.]] | [[Category: Varadarajan, R.]] | ||
| - | [[Category: NH2]] | ||
| - | [[Category: SO4]] | ||
[[Category: betaine]] | [[Category: betaine]] | ||
[[Category: osmolyte soaking]] | [[Category: osmolyte soaking]] | ||
| Line 33: | Line 34: | ||
[[Category: trimethylamine-n-oxide]] | [[Category: trimethylamine-n-oxide]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:29:51 2008'' |
Revision as of 18:29, 30 March 2008
| |||||||
| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Related: | IJ7Z, 1J80
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Osmolyte Stabilization of RNase
Overview
Osmolytes stabilize proteins to thermal and chemical denaturation. We have studied the effects of the osmolytes sarcosine, betaine, trimethylamine-N-oxide, and taurine on the structure and stability of the protein.peptide complex RNase S using x-ray crystallography and titration calorimetry, respectively. The largest degree of stabilization is achieved with 6 m sarcosine, which increases the denaturation temperatures of RNase S and S pro by 24.6 and 17.4 degrees C, respectively, at pH 5 and protects both proteins against tryptic cleavage. Four crystal structures of RNase S in the presence of different osmolytes do not offer any evidence for osmolyte binding to the folded state of the protein or any perturbation in the water structure surrounding the protein. The degree of stabilization in 6 m sarcosine increases with temperature, ranging from -0.52 kcal mol(-1) at 20 degrees C to -5.4 kcal mol(-1) at 60 degrees C. The data support the thesis that osmolytes that stabilize proteins, do so by perturbing unfolded states, which change conformation to a compact, folding competent state in the presence of osmolyte. The increased stabilization thus results from a decrease in conformational entropy of the unfolded state.
About this Structure
1J81 is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.
Reference
Osmolytes stabilize ribonuclease S by stabilizing its fragments S protein and S peptide to compact folding-competent states., Ratnaparkhi GS, Varadarajan R, J Biol Chem. 2001 Aug 3;276(31):28789-98. Epub 2001 May 23. PMID:11373282
Page seeded by OCA on Sun Mar 30 21:29:51 2008
