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5br1
From Proteopedia
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==CRYSTAL STRUCTURE OF AN ABC TRANSPORTER SOLUTE BINDING PROTEIN (IPR025997) FROM AGROBACTERIUM VITIS S4 (Avi_5305, TARGET EFI-511224) WITH BOUND ALPHA-D-GALACTOSAMINE== | ==CRYSTAL STRUCTURE OF AN ABC TRANSPORTER SOLUTE BINDING PROTEIN (IPR025997) FROM AGROBACTERIUM VITIS S4 (Avi_5305, TARGET EFI-511224) WITH BOUND ALPHA-D-GALACTOSAMINE== | ||
<StructureSection load='5br1' size='340' side='right' caption='[[5br1]], [[Resolution|resolution]] 1.85Å' scene=''> | <StructureSection load='5br1' size='340' side='right' caption='[[5br1]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=X6X:2-AMINO-2-DEOXY-ALPHA-D-GALACTOPYRANOSE'>X6X</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=X6X:2-AMINO-2-DEOXY-ALPHA-D-GALACTOPYRANOSE'>X6X</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5br1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5br1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5br1 RCSB], [http://www.ebi.ac.uk/pdbsum/5br1 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5br1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5br1 OCA], [http://pdbe.org/5br1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5br1 RCSB], [http://www.ebi.ac.uk/pdbsum/5br1 PDBsum]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The uptake of exogenous solutes by prokaryotes is mediated by transport systems embedded in the plasma membrane. In many cases, a solute-binding protein (SBP) is utilized to bind ligands with high affinity and deliver them to the membrane-bound components responsible for translocation into the cytoplasm. In the present study, Avi_5305, an Agrobacterium vitis SBP belonging to Pfam13407, was screened by differential scanning fluorimetry (DSF) and found to be stabilized by D-glucosamine and D-galactosamine. Avi_5305 is the first protein from Pfam13407 shown to be specific for amino sugars, and co-crystallization resulted in structures of Avi_5305 bound to D-glucosamine and D-galactosamine. Typical of Pfam13407, Avi_5305 consists of two alpha/beta domains linked through a hinge region, with the ligand-binding site located in a cleft between the two domains. Comparisons with Escherichia coli ribose-binding protein suggest that a cation-pi interaction with Tyr168 provides the specificity for D-glucosamine/D-galactosamine over D-glucose/D-galactose. | ||
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| + | Structure of an ABC transporter solute-binding protein specific for the amino sugars glucosamine and galactosamine.,Yadava U, Vetting MW, Al Obaidi N, Carter MS, Gerlt JA, Almo SC Acta Crystallogr F Struct Biol Commun. 2016 Jun 1;72(Pt 6):467-72. doi:, 10.1107/S2053230X16007500. Epub 2016 May 23. PMID:27303900<ref>PMID:27303900</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5br1" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
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[[Category: Enzyme function initiative]] | [[Category: Enzyme function initiative]] | ||
[[Category: Solute binding protein]] | [[Category: Solute binding protein]] | ||
| + | [[Category: Solute-binding protein]] | ||
[[Category: Structural genomic]] | [[Category: Structural genomic]] | ||
Revision as of 15:55, 23 June 2016
CRYSTAL STRUCTURE OF AN ABC TRANSPORTER SOLUTE BINDING PROTEIN (IPR025997) FROM AGROBACTERIUM VITIS S4 (Avi_5305, TARGET EFI-511224) WITH BOUND ALPHA-D-GALACTOSAMINE
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Categories: Almo, S C | Attonito, J D | Benach, J | Chamala, S | Chowdhury, S | EFI, Enzyme Function Initiative | Gerlt, J A | Glenn, A Scott | Lafleur, J | Love, J | Morisco, L L | Obaidi, N F.Al | Seidel, R D | Toro, R | Vetting, M W | Wasserman, S R | Whalen, K L | Yadava, U | Efi | Enzyme function initiative | Solute binding protein | Solute-binding protein | Structural genomic
