5j76
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Lectin from Colocasia esculenta(L.) Schott== | |
| + | <StructureSection load='5j76' size='340' side='right' caption='[[5j76]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5j76]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Colocasia_esculenta Colocasia esculenta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J76 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J76 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j76 OCA], [http://pdbe.org/5j76 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j76 RCSB], [http://www.ebi.ac.uk/pdbsum/5j76 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Mannose-binding beta-Prism Colocasia esculenta lectin (beta-PCL) was purified from tubers using ion exchange chromatography. The purified beta-PCL appeared as a single band of approximately 12kDa on SDS-PAGE. beta-PCL crystallizes in trigonal space group P3121 and diffracted to a resolution of 2.1A. The structure was solved using Molecular replacement using Crocus vernus lectin (PDB: 3MEZ) as a model. From the final refined model to an R-factor of 16.5% and an Rfree of 20.4%, it has been observed that the biological unit consists of two beta-Prism domains augmented through C-terminals swap over to form one of faces for each domain. Calpha superposition of individual domains of beta-PCL with individual domains of other related structures and superposition of whole protein structures were carried out. The higher RMS deviation for the superposition of whole structures suggest that beta-prism domains assume different orientation in each structure. | ||
| - | + | Structural analysis of beta-prism lectin from Colocasia esculenta (L.) S chott.,Vajravijayan S, Pletnev S, Pletnev VZ, Nandhagopal N, Gunasekaran K Int J Biol Macromol. 2016 Jun 1;91:518-523. doi: 10.1016/j.ijbiomac.2016.05.048. PMID:27262515<ref>PMID:27262515</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5j76" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Colocasia esculenta]] | ||
[[Category: Gunasekaran, K]] | [[Category: Gunasekaran, K]] | ||
[[Category: Nandhagopal, N]] | [[Category: Nandhagopal, N]] | ||
| + | [[Category: Pletnev, S]] | ||
| + | [[Category: Vajravijayan, S]] | ||
| + | [[Category: Colocasia agglutinin]] | ||
| + | [[Category: Lectin]] | ||
| + | [[Category: Sugar binding]] | ||
| + | [[Category: Sugar binding protein]] | ||
Revision as of 23:10, 23 June 2016
Structure of Lectin from Colocasia esculenta(L.) Schott
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