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5hft
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of HpxW== | |
| + | <StructureSection load='5hft' size='340' side='right' caption='[[5hft]], [[Resolution|resolution]] 2.65Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5hft]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HFT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HFT FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hft OCA], [http://pdbe.org/5hft PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hft RCSB], [http://www.ebi.ac.uk/pdbsum/5hft PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hft ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | HpxW from the ubiquitous pathogen Klebsiella pneumoniae is involved in a novel uric acid degradation pathway downstream from the formation of oxalurate. Specifically, HpxW is an oxamate amidohydrolase which catalyzes the conversion of oxamate to oxalate and is a member of the Ntn-hydrolase superfamily. HpxW is autoprocessed from an inactive precursor to form a heterodimer, resulting in a 35.5 kDa alpha subunit and a 20 kDa beta subunit. Here, the structure of HpxW is presented and the substrate complex is modeled. In addition, the steady-state kinetics of this enzyme and two active-site variants were characterized. These structural and biochemical studies provide further insight into this class of enzymes and allow a mechanism for catalysis consistent with other members of the Ntn-hydrolase superfamily to be proposed. | ||
| - | + | Biochemical and structural characterization of Klebsiella pneumoniae oxamate amidohydrolase in the uric acid degradation pathway.,Hicks KA, Ealick SE Acta Crystallogr D Struct Biol. 2016 Jun 1;72(Pt 6):808-16. doi:, 10.1107/S2059798316007099. Epub 2016 May 25. PMID:27303801<ref>PMID:27303801</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Ealick, S | + | <div class="pdbe-citations 5hft" style="background-color:#fffaf0;"></div> |
| - | [[Category: Hicks, K | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Ealick, S E]] | ||
| + | [[Category: Hicks, K A]] | ||
| + | [[Category: Amidohydrolase]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 10:24, 13 July 2016
Crystal structure of HpxW
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