1js2
From Proteopedia
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|PDB= 1js2 |SIZE=350|CAPTION= <scene name='initialview01'>1js2</scene>, resolution 1.90Å | |PDB= 1js2 |SIZE=350|CAPTION= <scene name='initialview01'>1js2</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR CLUSTER'>SF4</scene> | + | |LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1js2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1js2 OCA], [http://www.ebi.ac.uk/pdbsum/1js2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1js2 RCSB]</span> | ||
}} | }} | ||
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[[Category: Sundaralingam, M.]] | [[Category: Sundaralingam, M.]] | ||
[[Category: Xiong, Y.]] | [[Category: Xiong, Y.]] | ||
| - | [[Category: SF4]] | ||
[[Category: serine ligated [4fe-4s] cluster]] | [[Category: serine ligated [4fe-4s] cluster]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:38:14 2008'' |
Revision as of 18:38, 30 March 2008
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| , resolution 1.90Å | |||||||
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S} cluster
Overview
The crystal structure of Chromatium vinosum C77S HiPIP has been determined and is compared with that of wild type. This is the first reported crystal structure of a Ser ligated [4Fe-4S] cluster and reveals a 0.11 A shortening of the Fe-O bond (relative to Fe-S), but only minor structural alterations of the overall tertiary structure. Coordination changes are corroborated by resonance Raman spectroscopy. Comparison of the crystal and solution structures for HiPIPs identifies Phe48 as the main controller of solvent access to the Fe-S cluster; however, there is no significant change in cluster solvation of the C77S mutant relative to WT HiPIP. Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation.
About this Structure
1JS2 is a Single protein structure of sequence from Allochromatium vinosum. Full crystallographic information is available from OCA.
Reference
Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster., Mansy SS, Xiong Y, Hemann C, Hille R, Sundaralingam M, Cowan JA, Biochemistry. 2002 Jan 29;41(4):1195-201. PMID:11802718[[Category: serine ligated [4fe-4s] cluster]]
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