1jxg
From Proteopedia
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|PDB= 1jxg |SIZE=350|CAPTION= <scene name='initialview01'>1jxg</scene>, resolution 1.6Å | |PDB= 1jxg |SIZE=350|CAPTION= <scene name='initialview01'>1jxg</scene>, resolution 1.6Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene> | + | |LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jxg OCA], [http://www.ebi.ac.uk/pdbsum/1jxg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jxg RCSB]</span> | ||
}} | }} | ||
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[[Category: Milani, M.]] | [[Category: Milani, M.]] | ||
[[Category: Verbeet, M P.]] | [[Category: Verbeet, M P.]] | ||
| - | [[Category: CU1]] | ||
| - | [[Category: SO4]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:40:22 2008'' |
Revision as of 18:40, 30 March 2008
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| , resolution 1.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge
Overview
Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.
About this Structure
1JXG is a Single protein structure of sequence from Populus nigra. Full crystallographic information is available from OCA.
Reference
The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge., Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1735-8. Epub 2001, Oct 25. PMID:11679761
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