This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5jdb
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens== | |
| + | <StructureSection load='5jdb' size='340' side='right' caption='[[5jdb]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5jdb]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JDB FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jdb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jdb OCA], [http://pdbe.org/5jdb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jdb RCSB], [http://www.ebi.ac.uk/pdbsum/5jdb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jdb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/E2EA82_9REOV E2EA82_9REOV]] Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment.[SAAS:SAAS00136874] VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact.[SAAS:SAAS00136880] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | RotaTeq((R)) and Rotarix are two common human rotavirus (RV) vaccines currently on the market worldwide. Recent studies indicate histo-blood group antigens (HBGAs) may be attachment factors for RVs. The P[8] VP8* proteins of RotaTeq and Rotarix were expressed and purified, and their binding specificities were evaluated. Saliva-based binding assays showed that the VP8* proteins bound to the saliva samples of secretors irrespective of ABO blood types. However, in the oligosaccharide binding assay, the VP8* proteins displayed no specific binding to the HBGAs tested, including Lewis b and H1. The structure of RotaTeq P[8] VP8* was solved at 1.9A. Structural comparisons revealed that the putative receptor binding site was different to that of other genotypes and displayed a novel potential binding region. These findings indicate RotaTeq and Rotarix may have better efficiency in areas with a high percentage of secretors. | ||
| - | + | Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens.,Sun X, Guo N, Li D, Jin M, Zhou Y, Xie G, Pang L, Zhang Q, Cao Y, Duan ZJ Virology. 2016 Aug;495:129-35. doi: 10.1016/j.virol.2016.05.010. Epub 2016 May, 19. PMID:27209447<ref>PMID:27209447</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5jdb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cao, Y]] | ||
| + | [[Category: Duan, Z]] | ||
| + | [[Category: Guo, N]] | ||
| + | [[Category: Jin, M]] | ||
| + | [[Category: Li, D]] | ||
| + | [[Category: Pang, L]] | ||
| + | [[Category: Sun, X]] | ||
| + | [[Category: Xie, G]] | ||
| + | [[Category: Zhang, Q]] | ||
| + | [[Category: Zhou, Y]] | ||
| + | [[Category: Rotavirus]] | ||
| + | [[Category: Vaccine]] | ||
| + | [[Category: Viral protein]] | ||
| + | [[Category: Vp8]] | ||
Revision as of 11:46, 14 July 2016
Binding specificity of P[8] VP8* proteins of rotavirus vaccine strains with histo-blood group antigens
| |||||||||||
