5c8b
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | |||
==Structural insights into the redesign of a sucrose phosphorylase by induced loop repositioning== | ==Structural insights into the redesign of a sucrose phosphorylase by induced loop repositioning== | ||
<StructureSection load='5c8b' size='340' side='right' caption='[[5c8b]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='5c8b' size='340' side='right' caption='[[5c8b]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| Line 5: | Line 6: | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.7 2.4.1.7] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.7 2.4.1.7] </span></td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c8b OCA], [http://pdbe.org/5c8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c8b RCSB], [http://www.ebi.ac.uk/pdbsum/5c8b PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c8b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c8b OCA], [http://pdbe.org/5c8b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c8b RCSB], [http://www.ebi.ac.uk/pdbsum/5c8b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c8b ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Sucrose phosphorylases have been applied in the enzymatic production of glycosylated compounds for decades. However, several desirable acceptors, such as flavonoids or stilbenoids, that exhibit diverse antimicrobial, anticarcinogenic or antioxidant properties, remain poor substrates. The Q345F exchange in sucrose phosphorylase from Bifidobacterium adolescentis allows efficient glucosylation of resveratrol, (+)-catechin and (-)-epicatechin in yields of up to 97 % whereas the wild-type enzyme favours sucrose hydrolysis. Three previously undescribed products are made available. The crystal structure of the variant reveals a widened access channel with a hydrophobic aromatic surface that is likely to contribute to the improved activity towards aromatic acceptors. The generation of this channel can be explained in terms of a cascade of structural changes arising from the Q345F exchange. The observed mechanisms are likely to be relevant for the design of other tailor-made enzymes. | ||
| + | |||
| + | Redesign of the Active Site of Sucrose Phosphorylase through a Clash-Induced Cascade of Loop Shifts.,Kraus M, Grimm C, Seibel J Chembiochem. 2016 Jan 1;17(1):33-6. doi: 10.1002/cbic.201500514. Epub 2015 Dec 2. PMID:26527586<ref>PMID:26527586</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5c8b" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 06:20, 26 July 2016
Structural insights into the redesign of a sucrose phosphorylase by induced loop repositioning
| |||||||||||
