1k1q
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= DBH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]) | |GENE= DBH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1k1s|1K1S]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k1q OCA], [http://www.ebi.ac.uk/pdbsum/1k1q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k1q RCSB]</span> | ||
}} | }} | ||
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[[Category: lesion-bypass polymerase]] | [[Category: lesion-bypass polymerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:42:08 2008'' |
Revision as of 18:42, 30 March 2008
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| , resolution 2.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | DBH (Sulfolobus solfataricus) | ||||||
| Related: | 1K1S
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of a DinB Family Error Prone DNA Polymerase from Sulfolobus solfataricus
Overview
A new group of error-prone DNA polymerases overcomes the blockage posed to normal DNA replication by damaged template bases, suggesting an active site with a loose, flexible pocket that accommodates aberrant DNA structures. We have determined a 2.8 A resolution crystal structure of the Sulfolobus solfataricus Dbh protein, a DNA translesion polymerase closely related to Escherichia coli DNA polymerase IV and human polymerase kappa. A high error rate is observed for the Dbh polymerase in a range of 10(-2)-10(-3) for all 12 base substitution mispairs. The crystal structure of Dbh reveals an overall architecture resembling other DNA polymerases but has unique features that are likely to contribute to error-prone synthesis, including -1 frameshifting mutations.
About this Structure
1K1Q is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Crystal structure of a DinB family error-prone DNA polymerase from Sulfolobus solfataricus., Silvian LF, Toth EA, Pham P, Goodman MF, Ellenberger T, Nat Struct Biol. 2001 Nov;8(11):984-9. PMID:11685247
Page seeded by OCA on Sun Mar 30 21:42:08 2008
