1k4j
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= esaI/esaR cluster ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66271 Pantoea stewartii subsp. stewartii]) | |GENE= esaI/esaR cluster ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66271 Pantoea stewartii subsp. stewartii]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1kzf|1kzf]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k4j OCA], [http://www.ebi.ac.uk/pdbsum/1k4j PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k4j RCSB]</span> | ||
}} | }} | ||
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[[Category: Val, D L.]] | [[Category: Val, D L.]] | ||
[[Category: Watson, W T.]] | [[Category: Watson, W T.]] | ||
| - | [[Category: REO]] | ||
[[Category: acyl-homoserinelactone synthase]] | [[Category: acyl-homoserinelactone synthase]] | ||
[[Category: autoinducer synthase]] | [[Category: autoinducer synthase]] | ||
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[[Category: quorum sensing]] | [[Category: quorum sensing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:43:19 2008'' |
Revision as of 18:43, 30 March 2008
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| , resolution 2.5Å | |||||||
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| Ligands: | |||||||
| Gene: | esaI/esaR cluster (Pantoea stewartii subsp. stewartii) | ||||||
| Related: | 1kzf
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Acyl-homoserinelactone Synthase EsaI Complexed with Rhenate
Overview
Synthesis and detection of acyl-homoserine lactones (AHLs) enables many gram-negative bacteria to engage in quorum sensing, an intercellular signaling mechanism that activates differentiation to virulent and biofilm lifestyles. The AHL synthases catalyze acylation of S-adenosyl-L-methionine by acyl-acyl carrier protein and lactonization of the methionine moiety to give AHLs. The crystal structure of the AHL synthase, EsaI, determined at 1.8 A resolution, reveals a remarkable structural similarity to the N-acetyltransferases and defines a common phosphopantetheine binding fold as the catalytic core. Critical residues responsible for catalysis and acyl chain specificity have been identified from a modeled substrate complex and verified through functional analysis in vivo. A mechanism for the N-acylation of S-adenosyl-L-methionine by 3-oxo-hexanoyl-acyl carrier protein is proposed.
About this Structure
1K4J is a Single protein structure of sequence from Pantoea stewartii subsp. stewartii. Full crystallographic information is available from OCA.
Reference
Structural basis and specificity of acyl-homoserine lactone signal production in bacterial quorum sensing., Watson WT, Minogue TD, Val DL, von Bodman SB, Churchill ME, Mol Cell. 2002 Mar;9(3):685-94. PMID:11931774
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