1k5w
From Proteopedia
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|PDB= 1k5w |SIZE=350|CAPTION= <scene name='initialview01'>1k5w</scene> | |PDB= 1k5w |SIZE=350|CAPTION= <scene name='initialview01'>1k5w</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k5w OCA], [http://www.ebi.ac.uk/pdbsum/1k5w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k5w RCSB]</span> | ||
}} | }} | ||
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[[Category: Sudhof, T C.]] | [[Category: Sudhof, T C.]] | ||
[[Category: Ubach, J.]] | [[Category: Ubach, J.]] | ||
| - | [[Category: CA]] | ||
[[Category: c2-domain]] | [[Category: c2-domain]] | ||
[[Category: c2b-domain]] | [[Category: c2b-domain]] | ||
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[[Category: synaptotagmin i]] | [[Category: synaptotagmin i]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:43:55 2008'' |
Revision as of 18:43, 30 March 2008
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| Ligands: | |||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE
Overview
Synaptotagmin 1 probably functions as a Ca2+ sensor in neurotransmitter release via its two C2-domains, but no common Ca2+-dependent activity that could underlie a cooperative action between them has been described. The NMR structure of the C2B-domain now reveals a beta sandwich that exhibits striking similarities and differences with the C2A-domain. Whereas the bottom face of the C2B-domain has two additional alpha helices that may be involved in specialized Ca2+-independent functions, the top face binds two Ca2+ ions and is remarkably similar to the C2A-domain. Consistent with these results, but in contrast to previous studies, we find that the C2B-domain binds phospholipids in a Ca2+-dependent manner similarly to the C2A-domain. These results suggest a novel view of synaptotagmin function whereby the two C2-domains cooperate in a common activity, Ca2+-dependent phospholipid binding, to trigger neurotransmitter release.
About this Structure
1K5W is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine., Fernandez I, Arac D, Ubach J, Gerber SH, Shin O, Gao Y, Anderson RG, Sudhof TC, Rizo J, Neuron. 2001 Dec 20;32(6):1057-69. PMID:11754837
Page seeded by OCA on Sun Mar 30 21:43:55 2008
Categories: Rattus norvegicus | Single protein | Anderson, R G.W. | Arac, D. | Fernandez, I. | Gao, Y. | Gerber, S H. | Rizo, J. | Shin, O. | Sudhof, T C. | Ubach, J. | C2-domain | C2b-domain | Calcium-binding | Neurotransmitter release | Phospholipid-binding | Synapsis | Synaptic vesicle exocytosis | Synaptotagmin i
