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1k6g
From Proteopedia
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|PDB= 1k6g |SIZE=350|CAPTION= <scene name='initialview01'>1k6g</scene> | |PDB= 1k6g |SIZE=350|CAPTION= <scene name='initialview01'>1k6g</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1k6h|1K6H]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k6g OCA], [http://www.ebi.ac.uk/pdbsum/1k6g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k6g RCSB]</span> | ||
}} | }} | ||
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[[Category: rna tetraloop]] | [[Category: rna tetraloop]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:44:06 2008'' |
Revision as of 18:44, 30 March 2008
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| Ligands: | , , , | ||||||
| Related: | 1K6H
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA Processing
Overview
Rnt1p, the yeast orthologue of RNase III, cleaves rRNAs, snRNAs and snoRNAs at a stem capped with conserved AGNN tetraloop. Here we show that 9 bp long stems ending with AGAA or AGUC tetraloops bind to Rnt1p and direct specific but sequence-independent RNA cleavage when provided with stems longer than 13 bp. The solution structures of these two tetraloops reveal a common fold for the terminal loop stabilized by non-canonical A-A or A-C pairs and extensive base stacking. The conserved nucleotides are stacked at the 5' side of the loop, exposing their Watson-Crick and Hoogsteen faces for recognition by Rnt1p. These results indicate that yeast RNase III recognizes the fold of a conserved single-stranded tetraloop to direct specific dsRNA cleavage.
About this Structure
1K6G is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing., Lebars I, Lamontagne B, Yoshizawa S, Aboul-Elela S, Fourmy D, EMBO J. 2001 Dec 17;20(24):7250-8. PMID:11743001
Page seeded by OCA on Sun Mar 30 21:44:06 2008
