5dkl
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | |||
==Structure of the light-state dimer of the blue light photoreceptor Aureochrome 1a LOV from P. tricornutum== | ==Structure of the light-state dimer of the blue light photoreceptor Aureochrome 1a LOV from P. tricornutum== | ||
<StructureSection load='5dkl' size='340' side='right' caption='[[5dkl]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='5dkl' size='340' side='right' caption='[[5dkl]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
| Line 4: | Line 5: | ||
<table><tr><td colspan='2'>[[5dkl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DKL FirstGlance]. <br> | <table><tr><td colspan='2'>[[5dkl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DKL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5DKL FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dkl OCA], [http://pdbe.org/5dkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dkl RCSB], [http://www.ebi.ac.uk/pdbsum/5dkl PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5dkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dkl OCA], [http://pdbe.org/5dkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dkl RCSB], [http://www.ebi.ac.uk/pdbsum/5dkl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dkl ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The design of synthetic optogenetic tools that allow precise spatiotemporal control of biological processes previously inaccessible to optogenetic control has developed rapidly over the last years. Rational design of such tools requires detailed knowledge of allosteric light signaling in natural photoreceptors. To understand allosteric communication between sensor and effector domains, characterization of all relevant signaling states is required. Here, we describe the mechanism of light-dependent DNA binding of the light-oxygen-voltage (LOV) transcription factor Aureochrome 1a from Phaeodactylum tricornutum (PtAu1a) and present crystal structures of a dark state LOV monomer and a fully light-adapted LOV dimer. In combination with hydrogen/deuterium-exchange, solution scattering data and DNA-binding experiments, our studies reveal a light-sensitive interaction between the LOV and basic region leucine zipper DNA-binding domain that together with LOV dimerization results in modulation of the DNA affinity of PtAu1a. We discuss the implications of these results for the design of synthetic LOV-based photosensors with application in optogenetics. | ||
| + | |||
| + | Blue light-induced LOV domain dimerization enhances the affinity of Aureochrome 1a for its target DNA sequence.,Heintz U, Schlichting I Elife. 2016 Jan 12;5:e11860. doi: 10.7554/eLife.11860. PMID:26754770<ref>PMID:26754770</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5dkl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 08:11, 27 July 2016
Structure of the light-state dimer of the blue light photoreceptor Aureochrome 1a LOV from P. tricornutum
| |||||||||||
