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5k79
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k79 OCA], [http://pdbe.org/5k79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k79 RCSB], [http://www.ebi.ac.uk/pdbsum/5k79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k79 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k79 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k79 OCA], [http://pdbe.org/5k79 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k79 RCSB], [http://www.ebi.ac.uk/pdbsum/5k79 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k79 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The HIV-1 envelope glycoprotein gp120 is heavily glycosylated and bears numerous high-mannose sugars. These sugars can serve as targets for HIV-inactivating compounds, such as antibodies and lectins, which bind to the glycans and interfere with viral entry into the target cell. We determined the 1.6 A X-ray structure of Cyt-CVNH, a recently identified lectin from the cyanobacterium Cyanothece7424, and elucidated its glycan specificity by NMR. The Cyt-CVNH structure and glycan recognition profile are similar to those of other CVNH proteins, with each domain specifically binding to Mana(1-2)Mana units on the D1 and D3 arms of high-mannose glycans. However, in contrast to CV-N, no cross-linking and precipitation of the cross-linked species in solutions was observed upon Man-9 binding, allowing for the first time to investigate the interaction of Man-9 with a member of the CVNH family by NMR. HIV assays showed that Cyt-CVNH is able to inhibit HIV-1 with ~ 4-fold higher potency than CV-NP51G, a stabilized version of wild type CV-N. Therefore, Cyt-CVNH may qualify as a valuable lectin for potential microbicidal use. | ||
| + | |||
| + | Structure and Glycan Binding of a New Cyanovirin-N Homolog.,Matei E, Basu R, Furey W, Shi J, Calnan C, Aiken C, Gronenborn AM J Biol Chem. 2016 Jul 7. pii: jbc.M116.740415. PMID:27402833<ref>PMID:27402833</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5k79" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 08:11, 27 July 2016
Structure and anti-HIV activity of CYT-CVNH, a new cyanovirin-n homolog
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