5b0w
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the 11-cis isomer of pharaonis halorhodopsin in the absence of halide ions== | |
| + | <StructureSection load='5b0w' size='340' side='right' caption='[[5b0w]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5b0w]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis_dsm_2160 Natronomonas pharaonis dsm 2160]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B0W FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=22B:BACTERIORUBERIN'>22B</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=ILE:ISOLEUCINE'>ILE</scene>, <scene name='pdbligand=L3P:2,3-DI-O-PHYTANLY-3-SN-GLYCERO-1-PHOSPHORYL-3-SN-GLYCEROL-1-PHOSPHATE'>L3P</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qbg|3qbg]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0w OCA], [http://pdbe.org/5b0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b0w RCSB], [http://www.ebi.ac.uk/pdbsum/5b0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0w ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Like other microbial rhodopsins, the light driven chloride pump halorhodopsin from Natronomonas pharaonis (pHR) contains a mixture of all-trans/15-anti and 13-cis/15-syn isomers in the dark adapted state. A recent crystallographic study of the reaction states of pHR has shown that reaction states with 13-cis/15-syn retinal occur in the anion pumping cycle that is initiated by excitation of the all-trans isomer. In this study, we investigated interconversions among different isomeric states of pHR in the absence of chloride ions. The illumination of chloride free pHR with red light caused a large blue shift in the absorption maximum of the retinal visible band. During this "red adaptation", the content of the 11-cis isomer increased significantly, while the molar ratio of the 13-cis isomer to the all-trans isomer remained unchanged. The results suggest that the thermally activated interconversion between the 13-cis and the all-trans isomers is very rapid. Diffraction data from red adapted crystals showed that accommodation of the retinal chromophore with the 11-cis/15-syn configuration was achieved without a large change in the retinal binding pocket. The measurement of absorption kinetics under illumination showed that the 11-cis isomer, with a lambdamax at 565 nm, was generated upon excitation of a red-shifted species (lambdamax = 625 nm) that was present as a minor component in the dark adapted state. It is possible that this red-shifted species mimics an O-like reaction state with 13-cis/15-syn retinal, which was hypothesized to occur at a late stage of the anion pumping cycle. | ||
| - | + | Crystal Structure of the 11-cis Isomer of Pharaonis Halorhodopsin: Structural Constraints on Interconversions among Different Isomeric States.,Chan SK, Kawaguchi H, Kubo H, Murakami M, Ihara K, Maki K, Kouyama T Biochemistry. 2016 Jul 26;55(29):4092-104. doi: 10.1021/acs.biochem.6b00277. Epub, 2016 Jul 13. PMID:27352034<ref>PMID:27352034</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5b0w" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Natronomonas pharaonis dsm 2160]] | ||
| + | [[Category: Chan, S K]] | ||
[[Category: Kouyama, T]] | [[Category: Kouyama, T]] | ||
| - | [[Category: | + | [[Category: Light-driven chloride ion pump microbial rhodopsin]] |
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Retinylidene protein]] | ||
| + | [[Category: Seven trans-membrane helice]] | ||
Revision as of 15:34, 27 July 2016
Crystal structure of the 11-cis isomer of pharaonis halorhodopsin in the absence of halide ions
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