1kf1
From Proteopedia
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|PDB= 1kf1 |SIZE=350|CAPTION= <scene name='initialview01'>1kf1</scene>, resolution 2.1Å | |PDB= 1kf1 |SIZE=350|CAPTION= <scene name='initialview01'>1kf1</scene>, resolution 2.1Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=K:POTASSIUM ION'>K</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kf1 OCA], [http://www.ebi.ac.uk/pdbsum/1kf1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kf1 RCSB]</span> | ||
}} | }} | ||
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[[Category: Neidle, S.]] | [[Category: Neidle, S.]] | ||
[[Category: Parkinson, G N.]] | [[Category: Parkinson, G N.]] | ||
| - | [[Category: K]] | ||
[[Category: double chain reversal loop]] | [[Category: double chain reversal loop]] | ||
[[Category: g(anti).g(anti).g(anti)]] | [[Category: g(anti).g(anti).g(anti)]] | ||
| Line 31: | Line 33: | ||
[[Category: quadruplex dna]] | [[Category: quadruplex dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:47:37 2008'' |
Revision as of 18:47, 30 March 2008
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| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure and Packing of Human Telomeric DNA
Overview
Telomeric ends of chromosomes, which comprise noncoding repeat sequences of guanine-rich DNA, are fundamental in protecting the cell from recombination and degradation. Disruption of telomere maintenance leads to eventual cell death, which can be exploited for therapeutic intervention in cancer. Telomeric DNA sequences can form four-stranded (quadruplex) structures, which may be involved in the structure of telomere ends. Here we describe the crystal structure of a quadruplex formed from four consecutive human telomeric DNA repeats and grown at a K(+) concentration that approximates its intracellular concentration. K(+) ions are observed in the structure. The folding and appearance of the DNA in this intramolecular quadruplex is fundamentally different from the published Na(+)-containing quadruplex structures. All four DNA strands are parallel, with the three linking trinucleotide loops positioned on the exterior of the quadruplex core, in a propeller-like arrangement. The adenine in each TTA linking trinucleotide loop is swung back so that it intercalates between the two thymines. This DNA structure suggests a straightforward path for telomere folding and unfolding, as well as ways in which it can recognize telomere-associated proteins.
About this Structure
1KF1 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of parallel quadruplexes from human telomeric DNA., Parkinson GN, Lee MP, Neidle S, Nature. 2002 Jun 20;417(6891):876-80. Epub 2002 May 26. PMID:12050675
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