1kf8

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|SITE=
|SITE=
|LIGAND=
|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span>
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=[[1kf2|1KF2]], [[1kf3|1KF3]], [[1kf4|1KF4]], [[1kf5|1KF5]], [[1kf7|1KF7]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kf8 OCA], [http://www.ebi.ac.uk/pdbsum/1kf8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kf8 RCSB]</span>
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[[Category: titration]]
[[Category: titration]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:16:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:47:43 2008''

Revision as of 18:47, 30 March 2008

Image:1kf8.jpg


PDB ID 1kf8

Drag the structure with the mouse to rotate
, resolution 1.15Å
Activity: Pancreatic ribonuclease, with EC number 3.1.27.5
Related: 1KF2, 1KF3, 1KF4, 1KF5, 1KF7


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Atomic resolution structure of RNase A at pH 8.8


Overview

The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A.

About this Structure

1KF8 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829

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