5frh
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of oxidised RsrA== | |
| + | <StructureSection load='5frh' size='340' side='right' caption='[[5frh]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5frh]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FRH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FRH FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5frh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5frh OCA], [http://pdbe.org/5frh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5frh RCSB], [http://www.ebi.ac.uk/pdbsum/5frh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5frh ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/RSRA_STRCO RSRA_STRCO]] A redox-regulated anti-sigma factor for extracytoplasmic function (ECF) sigma factor SigR, and a key sensor of disulfide stress. Holds SigR, its cognate ECF sigma factor, in an inactive form, inhibiting its sigma activity under reducing but not oxidizing conditions; oxidation and reduction of the anti-sigma factor is reversible. Mycothiol (MSH) is competent for reduction of RsrA, allowing it to bind to SigR. In conjunction with its cognate sigma factor SigR may sense the intracellular level of reduced MSH. Probably releases SigR during oxidative stress.<ref>PMID:10428967</ref> <ref>PMID:11251822</ref> <ref>PMID:12381317</ref> <ref>PMID:14529630</ref> <ref>PMID:18430082</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Redox-regulated effector systems that counteract oxidative stress are essential for all forms of life. Here we uncover a new paradigm for sensing oxidative stress centred on the hydrophobic core of a sensor protein. RsrA is an archetypal zinc-binding anti-sigma factor that responds to disulfide stress in the cytoplasm of Actinobacteria. We show that RsrA utilizes its hydrophobic core to bind the sigma factor sigma(R) preventing its association with RNA polymerase, and that zinc plays a central role in maintaining this high-affinity complex. Oxidation of RsrA is limited by the rate of zinc release, which weakens the RsrA-sigma(R) complex by accelerating its dissociation. The subsequent trigger disulfide, formed between specific combinations of RsrA's three zinc-binding cysteines, precipitates structural collapse to a compact state where all sigma(R)-binding residues are sequestered back into its hydrophobic core, releasing sigma(R) to activate transcription of anti-oxidant genes. | ||
| - | + | The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core.,Rajasekar KV, Zdanowski K, Yan J, Hopper JT, Francis ML, Seepersad C, Sharp C, Pecqueur L, Werner JM, Robinson CV, Mohammed S, Potts JR, Kleanthous C Nat Commun. 2016 Jul 19;7:12194. doi: 10.1038/ncomms12194. PMID:27432510<ref>PMID:27432510</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5frh" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Kleanthous, C]] | [[Category: Kleanthous, C]] | ||
| - | [[Category: Potts, J | + | [[Category: Pecqueur, L]] |
| + | [[Category: Potts, J R]] | ||
[[Category: Werner, J]] | [[Category: Werner, J]] | ||
| + | [[Category: Zdanowski, K]] | ||
| + | [[Category: Anti-sigma factor]] | ||
| + | [[Category: Redox sensing]] | ||
| + | [[Category: Streptomyces coelicolor]] | ||
| + | [[Category: Transcription]] | ||
Revision as of 04:03, 4 August 2016
Solution structure of oxidised RsrA
| |||||||||||
